Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells

doi:10.1021/jacs.9b03498

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells
	Shen, JL ; Liang, L ; Xiao, MS ; Xie, XD ; Wang, F ; Li, Q ; Ge, ZL ; Li, J ; Shi, JY ; Wang, LH
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2019
卷号	141 期号:30 页码:11938-11946
关键词	RECEPTOR-MEDIATED ENDOCYTOSIS GOLD NANOPARTICLES PROTEIN CORONA DNA FUNCTIONALIZATION FIBRINOGEN SCATTERING ALBUMIN SHAPE
ISSN号	0002-7863
DOI	10.1021/jacs.9b03498
文献子类	期刊论文
英文摘要	The rapidly increasing need for systems biology stimulates the development of supermultiplex (SM) methods for simultaneously labeling multiple biomolecules/cells with distinct colors. Here we report the development of DNA-engineered fractal nanoplasmonic labels with ultrahigh brightness and photostability for SM imaging in single cells. These color-resolvable nanoplasmonic labels have a uniform size of similar to 50 nm with an inner hollow gap of similar to 1 nm. The outer shell morphology is highly tunable with the tip aspect ratio covering the range of delta = 0.29-1.66, which supports SM plasmonic imaging exceeding the conventional fluorescence multiplexing limit. We demonstrate the use of these SM labels for quantitative imaging of receptor-mediated endocytosis and intracellular transport of multiple protein-NP structures in a single cell in real time. This SM-plasmonic method sheds light on elucidating complex interactions among protein-NPs in nanotoxicology and facilitates the development of novel nanomedicines for diagnosis and therapy.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32069]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200240, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 201240, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol,CAS Key Lab Interfacial Phys & Tech, Shanghai 201800, Peoples R China; 4.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, Shanghai 200241, Peoples R China; 5.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Shen, JL,Liang, L,Xiao, MS,et al. Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(30):11938-11946.
APA	Shen, JL.,Liang, L.,Xiao, MS.,Xie, XD.,Wang, F.,...&Fan, CH.(2019).Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(30),11938-11946.
MLA	Shen, JL,et al."Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.30(2019):11938-11946.