Effect of lignin degradation product sinapyl alcohol on laccase catalysis during lignin degradation

doi:10.1016/j.indcrop.2019.111544

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Effect of lignin degradation product sinapyl alcohol on laccase catalysis during lignin degradation
	Zheng, YL ; Guo, M ; Zhou, QT ; Liu, HG
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2019
卷号	139 页码:-
关键词	BOVINE SERUM-ALBUMIN MOLECULAR DOCKING BINDING INTERACTION MECHANISM PROTEIN FUNGAL MODEL MEDIATORS BACTERIAL
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2019.111544
文献子类	期刊论文
英文摘要	Lignin is an excellent source of biomass; enzymatic hydrolysis of lignin is the key to its efficient use. The degradation activity of ligninase is affected by binding reactions with degradation products of lignin. In this paper, sinapyl alcohol (SA), a product of lignin degradation by laccase (LAC, a ligninase), was investigated as a mediator in enzymatic hydrolysis of lignin. This reaction was evaluated by molecular modelling, spectroscopic, dynamic light scattering, electrochemical experiments, gas chromatograph-mass spectrometer (GC-MS), nuclear magnetic resonance (NMR) and high-performance liquid chromatography (HPLC). These approaches allow elucidation of conformational changes in LAC caused by SA binding; confirm spontaneous binding between SA and LAC and electron transfer; determine the binding mode of SA with LAC; and enable determination of the mechanism of participation of the phenolic mediator SA in oxidation reactions after identification of 2,6-dimethoxy-1,4-benzoquinone (DMQ); besides, the effects of initial concentration of SA on the yields of DMQ; Our study shows how a small molecule mediator binds to LAC to form highly active intermediates, offering important insight into the processes involved in degradation of biomass.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32034]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, SSRF, Shanghai 201210, Peoples R China; 2.Zhejiang A&F Univ, Natl Engn & Technol Res Ctr Wood Based Resources, Hangzhou 311300, Zhejiang, Peoples R China; 3.Zhejiang A&F Univ, Dept Chem, Hangzhou 311300, Zhejiang, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Zheng, YL,Guo, M,Zhou, QT,et al. Effect of lignin degradation product sinapyl alcohol on laccase catalysis during lignin degradation[J]. INDUSTRIAL CROPS AND PRODUCTS,2019,139:-.
APA	Zheng, YL,Guo, M,Zhou, QT,&Liu, HG.(2019).Effect of lignin degradation product sinapyl alcohol on laccase catalysis during lignin degradation.INDUSTRIAL CROPS AND PRODUCTS,139,-.
MLA	Zheng, YL,et al."Effect of lignin degradation product sinapyl alcohol on laccase catalysis during lignin degradation".INDUSTRIAL CROPS AND PRODUCTS 139(2019):-.