Textured nanofibrils drive microglial phenotype

doi:10.1016/j.biomaterials.2020.120177

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	Textured nanofibrils drive microglial phenotype
	Song, Qin ; Pifferi, Simone ; Shi, Lin ; Chen, Chuntao ; Zaccaria, Remo Proietti ; Menini, Anna ; Cao, Jianping ; Zhang, Qi ; Torre, Vincent
刊名	BIOMATERIALS
	2020
卷号	257
关键词	BACTERIAL CELLULOSE CHANNEL EXPRESSION K+ PHYSIOLOGY MIGRATION HEALTH CELLS
DOI	10.1016/j.biomaterials.2020.120177
英文摘要	Microglia are highly plastic cells that change their properties in response to their microenvironment. By using immunofluorescence, live-cell imaging, electrophysiological recordings and RNA sequencing, we investigated the regulation of modified bacterial cellulose (mBC) nanofibril substrates on microglial properties. We demonstrate that mBC substrates induce ramified microglia with constantly extending and retracting processes, reminiscent of what is observed in vivo. Patch-clamp recordings show that microglia acquire a more negative resting membrane potential and have increased inward rectifier K+ currents, caused by an upregulation of Kir2.1 channels. Transcriptome analysis shows upregulation of genes involved in the immune response and down-regulation of genes linked to cell adhesion and cell motion. Furthermore, Arp2/3 complex activation and integrin-mediated signaling modulate microglial morphology and motility. Our studies demonstrate that mBC nanofibril substrates modulate microglial phenotype, paving the way for a microglia-material interface that may be very valuable for anti-neuroinflammatory drug screening.
学科主题	Engineering ; Materials Science
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20462]
专题	2020专题
作者单位	1.Zhang, Q 2.Torre, V (corresponding author), Int Sch Adv Studies SISSA, I-34136 Trieste, Italy. 3.Torre, V (corresponding author), Soochow Univ, State Key Lab Radiat Med & Protect, Med Coll, Sch Radiat Med & Protect, Suzhou, Jiangsu, Peoples R China.
推荐引用方式 GB/T 7714	Song, Qin,Pifferi, Simone,Shi, Lin,et al. Textured nanofibrils drive microglial phenotype[J]. BIOMATERIALS,2020,257.
APA	Song, Qin.,Pifferi, Simone.,Shi, Lin.,Chen, Chuntao.,Zaccaria, Remo Proietti.,...&Torre, Vincent.(2020).Textured nanofibrils drive microglial phenotype.BIOMATERIALS,257.
MLA	Song, Qin,et al."Textured nanofibrils drive microglial phenotype".BIOMATERIALS 257(2020).