Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity

doi:10.1007/s00284-020-01976-7

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所

	Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity
	Khadka Sujan 1,2,7; Adhikari Sanjib 1; Thapa Alina 3,7; Panday Raju 4; Adhikari Manjila 4; Sapkota Sanjeep 1,5,7; Regmi Ramesh Sharma 1; Adhikari Namita Paudel 3,7; Proshad Ram 6,7; Koirala Niranjan 8
刊名	CURRENT MICROBIOLOGY
	2020
页码	DOI: 10.1007/s00284-020-01976-7
ISSN号	0343-8651
DOI	10.1007/s00284-020-01976-7
通讯作者	Adhikari, Sanjib(sanadh26@gmail.com)
产权排序	2
文献子类	Article;Early Access
英文摘要	The current study was designed to isolate, identify and characterize a Bacillus sp. capable of producing protease and exhibiting antifungal activity. A highly potent bacterium capable of producing protease abundantly was isolated from the soil collected from the waste pit near Microbiology Laboratory of Birendra Multiple Campus, Bharatpur and later on identified as Lysinibacillus fusiformis strain SK on the basis of morphological, physiological, biochemical and 16S rDNA gene sequencing techniques. The strain SK showed 98.36% similarity with L. fusiformis strain NBRC 15717. Using R-programming statistical analysis tool, the optimum incubation time for the highest average protease production (APP) (47.2 U/mL) was found to be 22 h at 50 degrees C and both incubation time and temperature showed a significant impact on the production of protease (P < 0.01). The maximum average relative protease activity (ARPA) was observed at pH 7.8 and 48 degrees C, whereas the least ARPA was observed in the presence of 80 g/L NaCl and 10 g/L HgCl2 (P < 0.01). The newly isolated bacterial strain also exhibited strong antifungal activity against aflatoxigenic Aspergillus flavus and Aspergillus parasiticus suggesting that it can be a potential candidate for protease production and activity over a wider range of temperature and pH as well as for synthesizing effective antifungal compounds.
电子版国际标准刊号	1432-0991
WOS关键词	ASPERGILLUS-FLAVUS GENOMICS ; ALKALINE PROTEASE ; SP NOV. ; BACILLUS ; ITURIN ; OCHRATOXIN ; MYCOTOXINS
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000523426600002
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/34116]
专题	中国科学院水利部成都山地灾害与环境研究所
通讯作者	Adhikari Sanjib
作者单位	1.Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur 44200, Nepal; 2.State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 3.State Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China; 4.Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; 5.State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; 6.Key Laboratory of Mountain Surface Process and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China; 7.University of Chinese Academy of Sciences, Beijing 100049, China8)Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44613, Nepal; 8.Faculty of Science and Technology, University of Macau, Macau SAR 999078, China
推荐引用方式 GB/T 7714	Khadka Sujan,Adhikari Sanjib,Thapa Alina,et al. Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity[J]. CURRENT MICROBIOLOGY,2020:DOI: 10.1007/s00284-020-01976-7.
APA	Khadka Sujan.,Adhikari Sanjib.,Thapa Alina.,Panday Raju.,Adhikari Manjila.,...&Koirala Niranjan.(2020).Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity.CURRENT MICROBIOLOGY,DOI: 10.1007/s00284-020-01976-7.
MLA	Khadka Sujan,et al."Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity".CURRENT MICROBIOLOGY (2020):DOI: 10.1007/s00284-020-01976-7.