SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza

doi:10.1016/j.indcrop.2019.112004

	SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza
	Li, Wenrui 1,3; Xing, Bingcong 1,3; Mao, Renjun 4; Bai, Zhenqing 4; Yang, Dongfeng 2; Xu, Jiayi 4; Liang, Zongsuo 2,3
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2020-02-01
卷号	144 页码:11
关键词	Salvia mildorrhiza Tanshinones GRAS GA signaling Regulation
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2019.112004
通讯作者	Liang, Zongsuo(liangzs@ms.iswc.ac.cn)
英文摘要	Salvia miltiorrhiza Bunge is the origin plant of traditional Chinese medicine, Danshen. Demand of high-quality S. miltiorrhiza is increasing because of its excellent pharmaceutical value. Tanshinones and salvianolic acids (SAs) are the main active ingredients of S. miltiorrhiza. GA was reported to induce tanshinones and SAs accumulation, however, its mechanism was unclear. Here, we got a GA responsive protein, SmGRAS3. SmGRAS3 expressed highest in the periderm where tanshinones produced and accumulated. Results of SmGRAS3 over and antisense expression indicated that SmGRAS3 is a positive regulator of tanshinones biosynthesis in S. miltiorrhiza hairy roots. But overexpression of SmGRAS3 depressed SAs and GA biosynthesis as well as hairy roots growth, and application of GA could partially recover these depressions. Pathway enzyme genes expressions of tanshinones, SAs and GA biosynthesis were comprehensively changed in SmGRAS3 transgenic hairy roots. SmGRAS3 acted as a transcription factor that located in nucleus and has the transcription activation activity. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that SmGRAS3 directly bound to GARE-motif in promoter of KSL1 and activated KSL1 transcription. Our study suggested that the SmGRAS3 may be a good potential target for further metabolic engineering of bioactive components biosynthesis in S. miltiorrhiza.
资助项目	National Natural Science Foundation of China[81373908] ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program[2015BACO1B03]
WOS关键词	CHINESE MEDICINAL PREPARATIONS ; GRAS TRANSCRIPTION FACTOR ; HAIRY ROOT CULTURES ; PHENOLIC-ACIDS ; SYNTHASE GENE ; EXPRESSION ; ACCUMULATION ; SCARECROW ; PROTEINS ; INTERACTS
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000510521700010
资助机构	National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/132222]
专题	中国科学院地理科学与资源研究所
通讯作者	Liang, Zongsuo
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou 310018, Peoples R China 3.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China 4.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenrui,Xing, Bingcong,Mao, Renjun,et al. SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,144:11.
APA	Li, Wenrui.,Xing, Bingcong.,Mao, Renjun.,Bai, Zhenqing.,Yang, Dongfeng.,...&Liang, Zongsuo.(2020).SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza.INDUSTRIAL CROPS AND PRODUCTS,144,11.
MLA	Li, Wenrui,et al."SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza".INDUSTRIAL CROPS AND PRODUCTS 144(2020):11.