Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper

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	Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper
	Zhang, Chunsun ; Xu, Jinliang ; Wang, Jianqin ; Wang, Hanping
刊名	analytical letters
	2007-02-01
卷号	40 期号:3 页码:497-511
关键词	continuous-flow polymerase chain reaction microfluidics spiral capillary channel
通讯作者	xuil@ms.giec.ac.cn
中文摘要	this paper presents a novel method for performing polymerase chain reaction (pcr) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( ptfe) capillary tube was embedded. the channel with 25 pcr cycles was gradually developed in a spiral manner from inner to outer. the durations of pcr mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow pcr amplification as the synthesis ability of the taq polymerase enzyme usually weakens with pcr time. successful continuous-flow amplification of dna fragments has been demonstrated. the pcr products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of pcr mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. besides, the successful segmented-flow pcr of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow pcr microfluidics can be developed for high-throughput genetic analysis.
英文摘要	this paper presents a novel method for performing polymerase chain reaction (pcr) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( ptfe) capillary tube was embedded. the channel with 25 pcr cycles was gradually developed in a spiral manner from inner to outer. the durations of pcr mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow pcr amplification as the synthesis ability of the taq polymerase enzyme usually weakens with pcr time. successful continuous-flow amplification of dna fragments has been demonstrated. the pcr products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of pcr mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. besides, the successful segmented-flow pcr of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow pcr microfluidics can be developed for high-throughput genetic analysis.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	dna amplification ; through pcr ; reaction chip ; device ; system ; electrophoresis ; microchip ; time
收录类别	SCI
语种	英语
WOS记录号	WOS:000243120600008
公开日期	2010-09-27
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/3464]
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Micro Energy Syst Lab, Guangzhou 510640, Peoples R China 2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,et al. Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper[J]. analytical letters,2007,40(3):497-511.
APA	Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,&Wang, Hanping.(2007).Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper.analytical letters,40(3),497-511.
MLA	Zhang, Chunsun,et al."Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper".analytical letters 40.3(2007):497-511.