Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils

doi:10.1016/j.soilbio.2020.107938

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils
	Xiao, Leilei 1,2,3; Zheng, Shiling 2,3; Lichtfouse, Eric 5; Luo, Min 6; Tan, Yang 2; Liu, Fanghua 1,2,3,4
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2020-11-01
卷号	150 页码:9
关键词	Methane DIET-Independent methanogenesis Conductive material Acetoclastic methanogenesis Pure culture Proteome
ISSN号	0038-0717
DOI	10.1016/j.soilbio.2020.107938
通讯作者	Liu, Fanghua(fhliu@yic.ac.cn)
英文摘要	Direct interspecies electron transfer (DIET) between electricigens and methanogens has been shown to favour CO2 reduction to produce biomethane. Furthermore, DIET is accelerated by conductive materials. However, whether conductive materials can promote other methanogenic pathways is unclear due to a lack of detailed experimental data and the poor mechanistic studies. Here, we hypothesized that conductive carbon nanotubes (CNTs) stimulate acetoclastic methanogenesis independently of electricigens in pure cultures of Methanosarcina spp. and anaerobic wetland soil. We found a significant increase in the methane production rate during the growth phase, e.g. from 0.169 mM to 0.241 mM after addition of CNTs on the 3rd day. CNTs did not increase the abundance of electromicroorganisms or the electron transfer rate in anaerobic soils, using via microbial diversity and electrochemical analysis. C-13-CH3COOH labelling, stable carbon isotope fractionation and the CH3F inhibitor of acetoclastic methanogenesis were used to distinguish methanogenic pathways. CNTs mainly accelerated acetoclastic methanogenesis rather than CO2 reduction in both pure cultures and anaerobic soils. Furthermore, the presence of CNTs slightly alleviate the inhibition of CH3F on acetoclastic methanogenesis during the pure culture of Methanosarcina barkeri and Methanosarcina mazei with the production of more than 0.3 mM methane. CNTs closely attached to the cell surface were observed by transmission electron microscopy. Proteome analysis revealed a stimulation of protein synthesis with about twice the improvement involved in -COOH oxidation and electron transfer. Overall, our findings demonstrate that conducting CNTs favor methane production and that the mechanism involved is acetoclastic methanogenesis via acetate dismutation, at least partly, rather than classical CO2 reduction.
资助项目	National Natural Science Foundation of China[41703075] ; National Natural Science Foundation of China[91751112] ; National Natural Science Foundation of China[41573071] ; Natural Science Foundation of Shandong Province[JQ201608] ; Natural Science Foundation of Shandong Province[ZR2016DQ12] ; Young Taishan Scholars Program of Shandong Province[tsqn20161054]
WOS关键词	INTERSPECIES ELECTRON-TRANSFER ; METHANE PRODUCTION ; ISOTOPE FRACTIONATION ; ORGANIC-MATTER ; PADDY SOIL ; GROWTH ; CO2 ; COMMUNITIES ; BACTERIAL ; ACETATE
WOS研究方向	Agriculture
语种	英语
WOS记录号	WOS:000581509300005
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Young Taishan Scholars Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28452]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Liu, Fanghua
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Aix Marseille Univ, Coll France, CEREGE, INRAE,IRD,CNRS, Ave Louis Philibert, F-13100 Aix En Provence, France 6.Fuzhou Univ, Sch Environm & Resource, Fuzhou 350116, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Leilei,Zheng, Shiling,Lichtfouse, Eric,et al. Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils[J]. SOIL BIOLOGY & BIOCHEMISTRY,2020,150:9.
APA	Xiao, Leilei,Zheng, Shiling,Lichtfouse, Eric,Luo, Min,Tan, Yang,&Liu, Fanghua.(2020).Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils.SOIL BIOLOGY & BIOCHEMISTRY,150,9.
MLA	Xiao, Leilei,et al."Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils".SOIL BIOLOGY & BIOCHEMISTRY 150(2020):9.