The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure

doi:10.1007/s10570-020-03087-9

	The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure
	Chen, Xindong 1,2; Xiong, Lian 1,3,4,5; Li, Hailong 1,3,4,5; Zhang, Liquan 1,2; Yuan, Ge 1,2; Chen, Xuefang 1,3,4,5; Wang, Can 1,3,4,5; Chen, Xinde 1,3,4,5
刊名	CELLULOSE
	2020-05-01
卷号	27 期号:8 页码:4417-4428
关键词	Cellulose Xylan inhibition Enzymatic hydrolysis Cellulase
ISSN号	0969-0239
DOI	10.1007/s10570-020-03087-9
通讯作者	Li, Hailong(lihl@ms.giec.ac.cn) ; Chen, Xinde(cxd_cxd@hotmail.com)
英文摘要	The inhibitory effect of xylan on enzymatic hydrolysis of cellulose with different ultrastructure is not clear. In this work, cellulose II, cellulose III, and amorphous cellulose were prepared from cellulose I by NaOH, ethylenediamine, and ball-milling treatment, respectively. Their crystalline structure and enzymatic hydrolyzability were systematically characterized and compared with cellulose I. Furthermore, the inhibitory effect of xylan on cellulose with different ultrastructure was investigated in detail. Results suggested that the enzymatic hydrolyzability of different cellulose increased in sequence from cellulose I (25.4%), cellulose III (41.7%), cellulose II (56.2%) to amorphous cellulose (77.4%) at enzyme loadings of 5.56 mg protein/g substrate. The enzymatic hydrolyzability of allomorphic cellulose was strongly inhibited by xylan, while a little inhibition was observed for amorphous cellulose. It was found that cellobiose released from different cellulose was decreased after the addition of xylan. At the initial hydrolysis stage, the inhibition degree was obviously increased for all of different cellulose, and then, the inhibition degree of cellulose I and cellulose II gradually decreased as enzymatic hydrolysis proceeded, while the inhibition degree of cellulose III and amorphous cellulose had changed a little. In addition, at a longer time of hydrolysis, the inhibition degree was decreased with the increase of cellulase dosage except for cellulose III, which remained approximately unchanged. Overall, this study demonstrated that the inhibitory effect of xylan on cellulose hydrolysis was substantially affected by the ultrastructure of cellulose, and thus it provides new insights for relieving the inhibitory effect of xylan by altering the ultrastructure of cellulose.
WOS关键词	AMMONIA PRETREATMENT ; ENZYMES ; LIGNOCELLULOSE ; LIGNIN ; RECALCITRANCE ; EFFICIENCY ; CONVERSION ; ALLOMORPH
WOS研究方向	Materials Science ; Polymer Science
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000528396300018
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/26800]
专题	中国科学院广州能源研究所
通讯作者	Li, Hailong; Chen, Xinde
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Energy Rd, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 5.Chinese Acad Sci, R&D Ctr Xuyi Attapulgite Appl Technol, Guangzhou Inst Energy Convers, Xuyi 211700, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xindong,Xiong, Lian,Li, Hailong,et al. The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure[J]. CELLULOSE,2020,27(8):4417-4428.
APA	Chen, Xindong.,Xiong, Lian.,Li, Hailong.,Zhang, Liquan.,Yuan, Ge.,...&Chen, Xinde.(2020).The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure.CELLULOSE,27(8),4417-4428.
MLA	Chen, Xindong,et al."The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure".CELLULOSE 27.8(2020):4417-4428.