Enhanced enzymatic hydrolysis of wheat straw via a combination of alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide pretreatment

doi:10.1016/j.indcrop.2019.05.027

	Enhanced enzymatic hydrolysis of wheat straw via a combination of alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide pretreatment
	Li, Hailong 1,2,3,4; Xiong, Lian 1,2,3,4; Chen, Xindong 2,5; Luo, Mutan 2,5; Chen, Xuefang 1,2,3,4; Wang, Can 1,2,3,4; Huang, Chao 1,2,3,4; Chen, Xinde 1,2,3,4
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2019-10-01
卷号	137 页码:332-338
关键词	Alkaline hydrogen peroxide Lithium chloride/N,N-dimethylacetamide Pretreatment Enzymatic hydrolysis Synergistic effect
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2019.05.027
通讯作者	Chen, Xinde(cxd_cxd@hotmail.com)
英文摘要	The aim of this work was to enhance enzymatic hydrolysis of wheat straw (WS) via a combination of alkaline hydrogen peroxide (AHP) and lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) pretreatment. The physicochemical structural changes of pretreated WS were systematically characterized. Furthermore, the hydrolytic efficiency and synergistic action between xylanase and cellulase in the enzymatic hydrolysis of pretreated WS were investigated. Results suggested that AHP-LiCl/DMAc pretreatment enhanced the enzymatic hydrolysis efficiency dramatically by effective delignification and completely deconstructing the crystalline structure of cellulose in WS. The maximum cellulose and xylan enzymatic hydrolysis efficiencies of AHP-LiCl/DMAc treated WS reached up to 97.49% and 87.80%, respectively, by adding cellulase and xylanase simultaneously. The disruption of hydrogen bond in cellulose and xylan reduced the synergistic effect between xylanase and cellulase during enzymatic hydrolysis of AHP-LiCl/DMAc pretreated WS, especially for cellulose hydrolysis. The hydrogen bond present in cellulose and xylan is one of the key factors on determining the synergistic action of xylanase and cellulase, which also plays an important role in enzymatic saccharification process. Overall, this study provides a promising pretreatment strategy for the efficient enzymatic hydrolysis of WS into fermentable sugars.
资助项目	National Natural Science Foundation of China[31600475] ; Natural Science Foundation of Guangdong Province[2017A030310052] ; Natural Science Foundation of Guangdong Province[2016A030310124] ; project of Guangzhou Science and Technology[201707010241] ; Key Research and Development Plan of Jiangsu Province[BE2016706]
WOS关键词	CELLULOSE ACCESSIBILITY ; BIOMASS ; MECHANISM ; DIGESTIBILITY ; DISSOLUTION ; CONVERSION ; XYLANASE ; ENZYMES ; SUGARS ; WASTE
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000473376800041
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; project of Guangzhou Science and Technology ; Key Research and Development Plan of Jiangsu Province
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/25381]
专题	中国科学院广州能源研究所
通讯作者	Chen, Xinde
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi 211700, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Hailong,Xiong, Lian,Chen, Xindong,et al. Enhanced enzymatic hydrolysis of wheat straw via a combination of alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide pretreatment[J]. INDUSTRIAL CROPS AND PRODUCTS,2019,137:332-338.
APA	Li, Hailong.,Xiong, Lian.,Chen, Xindong.,Luo, Mutan.,Chen, Xuefang.,...&Chen, Xinde.(2019).Enhanced enzymatic hydrolysis of wheat straw via a combination of alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide pretreatment.INDUSTRIAL CROPS AND PRODUCTS,137,332-338.
MLA	Li, Hailong,et al."Enhanced enzymatic hydrolysis of wheat straw via a combination of alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide pretreatment".INDUSTRIAL CROPS AND PRODUCTS 137(2019):332-338.