Effect of Pyrethrins and Rosemary on the ATPase Activities  of Rat Cerebral Synaptosome

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	Effect of Pyrethrins and Rosemary on the ATPase Activities of Rat Cerebral Synaptosome
	Liu ZC 1,2; Liang XC*2; liangxc@mail.kiz.ac.cn
刊名	应用与环境生物学报
	2008
卷号	14 期号:3 页码:399-402
关键词	除虫菊酯迷迭香总atp酶 mg2+-atp酶大鼠大脑突触体
其他题名	除虫菊酯和迷迭香对大鼠大脑突触体ATP酶的活性影响
英文摘要	Pyrethrins are the widely used insecticides in agriculture and households, and they act on integral membrane proteins, causing neurotoxic effects on animals. Rosemary, as an antioxidant, is developed to improve the stability of pyrethrins and applied for many commercially pyrethrin-related products. This paper aimed to study the effects of pyrethrins and rosemary, as well as their combination on the cerebral synaptosome ATPase of rat. The Percoll gradient method was used to isolate synaptosomes. Total ATPase and Mg2＋ -ATPase activities were analyzed by determining the amount of inorganic phosphate. The results indicated that the activities of total ATPase and Mg2＋ -ATPase in 10 μmoL/L pyrethrins were reduced to 80.3% and 46. 9% , respectively, compared with CK. However, rosemary at concentration of 0.3 to 30 μmoL/L showed little effect on AT- Pase, while at the concentration of 3 000 μmoL/L, the total ATPase and the Mg2＋ -ATPase activities were decreased to 66.8% and 54.5% , respectively. The mixture of 10 μmoL/L pyrethrin and 30 μmoL/L rosemary decreased the activities of the total ATPase and Mg2＋ -ATPase to 72.9% and 33.4% , respectively. It is concluded that pyrethrins can inhibit ATPase activity of rat cerebral synaptosome dramatically; rosemary inhibits ATPase only at high concentration; and rosemary slightly enhances inhibition of pyrethrins on ATPase activity.
语种	英语
资助机构	Acknowledgements We thank Honghe Senju Biology Co. , for providing us with pyrethrins and rosemary. We also thank Dr. LI Xueyan for her helpful discussion and reviewing this manuscript. ; Acknowledgements We thank Honghe Senju Biology Co. , for providing us with pyrethrins and rosemary. We also thank Dr. LI Xueyan for her helpful discussion and reviewing this manuscript. ; Acknowledgements We thank Honghe Senju Biology Co. , for providing us with pyrethrins and rosemary. We also thank Dr. LI Xueyan for her helpful discussion and reviewing this manuscript. ; Acknowledgements We thank Honghe Senju Biology Co. , for providing us with pyrethrins and rosemary. We also thank Dr. LI Xueyan for her helpful discussion and reviewing this manuscript.
公开日期	2010-08-05
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/936]
专题	昆明动物研究所_其他
通讯作者	liangxc@mail.kiz.ac.cn
作者单位	1.中国科学院研究生院;北京100049 2.中国科学院昆明动物研究所;昆明650223
推荐引用方式 GB/T 7714	Liu ZC,Liang XC*,liangxc@mail.kiz.ac.cn. Effect of Pyrethrins and Rosemary on the ATPase Activities of Rat Cerebral Synaptosome[J]. 应用与环境生物学报,2008,14(3):399-402.
APA	Liu ZC,Liang XC*,&liangxc@mail.kiz.ac.cn.(2008).Effect of Pyrethrins and Rosemary on the ATPase Activities of Rat Cerebral Synaptosome.应用与环境生物学报,14(3),399-402.
MLA	Liu ZC,et al."Effect of Pyrethrins and Rosemary on the ATPase Activities of Rat Cerebral Synaptosome".应用与环境生物学报 14.3(2008):399-402.