茎瘤固氮根瘤菌环二鸟苷酸代谢相关基因的功能鉴定 | |
杨学智; 沈日敏; 孙雨; 李润植; 解志红 | |
刊名 | 微生物学报
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2019 | |
卷号 | 59期号:8页码:1500-1511 |
关键词 | 茎瘤固氮根瘤菌ors571 环化酶dgc 环二鸟苷酸c-di-gmp 胞外多糖 生物膜 Azorhizobium Caulinodans Ors571 Diguanylate Cyclase C-di-gmp Exopolysaccharide Biofilm |
ISSN号 | 0001-6209 |
其他题名 | Characterization of diguanylate cyclase metabolism-related gene in Azorhizobium caulinodans ORS571 |
产权排序 | (1) 山西农业大学农学院 ; (2) 中国科学院烟台海岸带研究所 |
英文摘要 | [Objective] To study the phenotypes of Azorhizobium caulinodansORS571 mutant strain lacking the diguanylate cyclase AZC-2412, and to explore its functional mechanism. [Methods] We constructed the gene knockout system of rhizobia based on the homologous recombination of cre-loxp, and the mutant strain was constructed by the triparental conjugation. Growth rate, chemotactic ability, exopolysaccharide production, biofilm formation and other phenotypes of the wild type and mutant strains were studied. [Results] The mutant strain had almost the same growth rate as the wild type. Compared with the wild type, the mutant strain impaired in exopolysaccharide production, chemotaxis behavior and biofilm formation due to lower levels of c-di-GMP in cells. [Conclusion] The deletion of the diguanylate cyclase AZC-2412 reduces level of c-di-GMP levels. It had a regulatory effect on the exopolysaccharide production, bacterial motility, biofilm formation, cell flocculation of Azorhizobium caulinodans ORS571 and its interaction with host plant. |
WOS关键词 | 茎瘤固氮根瘤菌ors571 ; 环化酶dgc ; 环二鸟苷酸c-di-gmp ; 胞外多糖 ; 生物膜 ; Azorhizobium Caulinodans Ors571 ; Diguanylate Cyclase ; C-di-gmp ; Exopolysaccharide ; Biofilm |
WOS研究方向 | Microbiology |
语种 | 中文 |
CSCD记录号 | CSCD:6544329 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/25146] ![]() |
专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.山西农业大学农学院; 2.中国科学院烟台海岸带研究所 |
推荐引用方式 GB/T 7714 | 杨学智,沈日敏,孙雨,等. 茎瘤固氮根瘤菌环二鸟苷酸代谢相关基因的功能鉴定[J]. 微生物学报,2019,59(8):1500-1511. |
APA | 杨学智,沈日敏,孙雨,李润植,&解志红.(2019).茎瘤固氮根瘤菌环二鸟苷酸代谢相关基因的功能鉴定.微生物学报,59(8),1500-1511. |
MLA | 杨学智,et al."茎瘤固氮根瘤菌环二鸟苷酸代谢相关基因的功能鉴定".微生物学报 59.8(2019):1500-1511. |
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