茎瘤固氮根瘤菌ORS571 GGDEF和EAL结构域蛋白的预测及功能研究 | |
Sun Yu; Xie Zhihong; Liu Wei; Guo Hongen | |
刊名 | 微生物学报
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2019 | |
卷号 | 59期号:10页码:2000-2012 |
关键词 | 茎瘤固氮根瘤菌ors571 环二鸟苷酸 Ggdef/eal结构域 Azorhizobium Caulinodans Ors571 C-di-gmp Ggdef/eal Domain |
ISSN号 | 0001-6209 |
其他题名 | Prediction and functional analysis of GGDEF/EAL domaincontaining proteins in Azorhizobium caulinodans ORS571 |
产权排序 | (1) 中国科学院烟台海岸带研究所 ; (2) 中国科学院大学 ; (3) 山东省蚕业研究所 |
英文摘要 | [Objective] c-di-GMP,an important second messenger regulating multiple functions of bacteria,is generally synthesized and hydrolysed by proteins containing GGDEF or EAL domain.In this study,we analyzed the genome-wide GGDEF/EAL domain-containing proteins of Azorhizobium caulinodans ORS571,and selected three GGDEF-EAL composite proteins (AZC_3085,AZC_3226 and AZC_4658) for functional analysis.[Methods] SMART and CLUSTALW were used for prediction and multi-alignment of GGDEF/EAL domain-containing proteins.Mutants were constructed by homologous recombination.Phenotypes including cell motility,exopolysaccharide (EPS) production,biofilm formation and nodulation with legume host were investigated.[Results] There were 37 GGDEF/EAL domain-containing proteins in A.caulinodans ORS571.Mutant Delta4658 showed deficiency in cell motility,while its EPS production and biofilm formation were higher than that of wild type.Mutant Delta4658 showed stronger competitiveness than wild type in competitive nodulation assay.The loss of AZC_4658 led to the increase of intracellular c-di-GMP level.Mutants Delta3085 and Delta3226 did not show obvious difference in comparison with wild type.[Conclusion] The vast number of GGDEF/EAL domain-containing proteins suggested that c-di-GMP may play an important role in signal transduction of ORS571.The GGDEF-EAL composite protein AZC_4658 was involved in cell motility,EPS production,biofilm formation and nodulation of A.caulinodans ORS571. |
WOS关键词 | 茎瘤固氮根瘤菌ors571 ; 环二鸟苷酸 ; Ggdef/eal结构域 ; Azorhizobium Caulinodans Ors571 ; C-di-gmp ; Ggdef/eal Domain |
WOS研究方向 | Microbiology |
语种 | 中文 |
CSCD记录号 | CSCD:6575584 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/25137] ![]() |
专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.中国科学院烟台海岸带研究所; 2.中国科学院大学; 3.山东省蚕业研究所 |
推荐引用方式 GB/T 7714 | Sun Yu,Xie Zhihong,Liu Wei,等. 茎瘤固氮根瘤菌ORS571 GGDEF和EAL结构域蛋白的预测及功能研究[J]. 微生物学报,2019,59(10):2000-2012. |
APA | Sun Yu,Xie Zhihong,Liu Wei,&Guo Hongen.(2019).茎瘤固氮根瘤菌ORS571 GGDEF和EAL结构域蛋白的预测及功能研究.微生物学报,59(10),2000-2012. |
MLA | Sun Yu,et al."茎瘤固氮根瘤菌ORS571 GGDEF和EAL结构域蛋白的预测及功能研究".微生物学报 59.10(2019):2000-2012. |
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