Identification of Cbp1, a c-di-GMP Binding Chemoreceptor in Azorhizobium caulinodans ORS571 Involved in Chemotaxis and Nodulation of the Host Plant

doi:10.3389/fmicb.2019.00638

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Identification of Cbp1, a c-di-GMP Binding Chemoreceptor in Azorhizobium caulinodans ORS571 Involved in Chemotaxis and Nodulation of the Host Plant
	Sun, Y ; Xie, ZH ; Sui, F ; Liu, XL ; Cheng, WZ
刊名	FRONTIERS IN MICROBIOLOGY
	2019-04-02
卷号	10 页码:638
关键词	c-di-GMP PilZ domain chemoreceptor chemotaxis nodulation
ISSN号	1664-302X
DOI	10.3389/fmicb.2019.00638
产权排序	[Sun, Yu ; Xie, Zhihong ; Sui, Fu ; Liu, Xiaolin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China ; [Sun, Yu ; Sui, Fu ; Liu, Xiaolin] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China ; [Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China ; [Cheng, Wuzeng] Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China
文献子类	Article
英文摘要	Cbp1, a chemoreceptor containing a PilZ domain was identified in Azorhizobium caulinodans ORS571, a nitrogen-fixing free-living soil bacterium that induces nodule formation in both the roots and stems of the host legume Sesbania rostrata. Chemoreceptors are responsible for sensing signals in the chemotaxis pathway, which guides motile bacteria to beneficial niches and plays an important role in the establishment of rhizobia-legume symbiosis. PilZ domain proteins are known to bind the second messenger c-di-GMP, an important regulator of motility, biofilm formation and virulence. Cbp1 was shown to bind c-di-GMP through the conserved RxxxR motif of its PilZ domain. A mutant strain carrying a cbp1 deletion was impaired in chemotaxis, a feature that could be restored by genetic complementation. Compared with the wild type strain, the Delta cbp1 mutant displayed enhanced aggregation and biofilm formation. The Delta cbp1 mutant induced functional nodules when inoculated individually. However, the Delta cbp1 mutant was less competitive than the wild type in competitive root colonization and nodulation. These data are in agreement with the hypothesis that the c-di-GMP binding chemoreceptor Cbp1 in A. caulinodans is involved in chemotaxis and nodulation.
WOS关键词	PILZ DOMAIN PROTEINS ; CYCLIC DIGUANYLATE ; BACTERIAL CHEMOTAXIS ; SIGNAL-TRANSDUCTION ; ALLOSTERIC CONTROL ; STRUCTURAL BASIS ; RECEPTOR ; PHOSPHODIESTERASE ; AGGREGATION ; MECHANISM
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000463046600001
资助机构	NSFC-Shandong Joint Fund Key Projects [U1806206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31570063, 31870020] ; Mechanisms of Marginal Land Productivity Expansion and Technologies of Storing Grain in Land [KFZD-SW-112] ; Shandong Key Research and Development Program [2017GSF17129] ; Shandong Key Scientific and Technological Innovation Program [2017CXGC0303]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24961]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室中国科学院烟台海岸带研究所
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China; 4.Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China
推荐引用方式 GB/T 7714	Sun, Y,Xie, ZH,Sui, F,et al. Identification of Cbp1, a c-di-GMP Binding Chemoreceptor in Azorhizobium caulinodans ORS571 Involved in Chemotaxis and Nodulation of the Host Plant[J]. FRONTIERS IN MICROBIOLOGY,2019,10:638.
APA	Sun, Y,Xie, ZH,Sui, F,Liu, XL,&Cheng, WZ.(2019).Identification of Cbp1, a c-di-GMP Binding Chemoreceptor in Azorhizobium caulinodans ORS571 Involved in Chemotaxis and Nodulation of the Host Plant.FRONTIERS IN MICROBIOLOGY,10,638.
MLA	Sun, Y,et al."Identification of Cbp1, a c-di-GMP Binding Chemoreceptor in Azorhizobium caulinodans ORS571 Involved in Chemotaxis and Nodulation of the Host Plant".FRONTIERS IN MICROBIOLOGY 10(2019):638.