Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium

doi:10.1007/s00253-019-09994-7

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	Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium
	Min, J ; Xu, LX ; Fang, SY ; Chen, WW ; Hu, XK
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2019-09
卷号	103 期号:18 页码:7741-7750
关键词	1 2 4-Benzenetriol pathway 2-Chloro-4-nitrophenol Catabolic mechanism Gram-negative bacterium hnp cluster Two-component monooxygenase
ISSN号	0175-7598
DOI	10.1007/s00253-019-09994-7
产权排序	[Min, Jun ; Xu, Lingxue ; Fang, Suyun ; Chen, Weiwei ; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China ; [Min, Jun ; Fang, Suyun ; Chen, Weiwei ; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China ; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China
文献子类	Article
英文摘要	2-Chloro-4-nitrophenol (2C4NP) is the most common chlorinated nitrophenol pollutant, and its environmental fate is of great concern. Cupriavidus sp. CNP-8, a Gram-negative bacterium, has been reported to degrade 2C4NP via the 1,2,4-benzenetriol (BT) pathway, significantly different from the (chloro)hydroquinone pathways reported in all other Gram-negative 2C4NP-utilizers. Herein, the BT pathway of the catabolism of 2C4NP in this strain was characterized at the molecular, biochemical, and genetic levels. The hnp gene cluster was suspected to be involved in the catabolism of 2C4NP because the hnp genes are significantly upregulated in the 2C4NP-induced strain CNP-8 compared to the uninduced strain. HnpAB, a two-component FAD-dependent monooxygenase, catalyzes the conversion of 2C4NP to BT via chloro-1,4-benzoquinone, with a K-m of 2.7 +/- 1.1 mu M and a k(cat)/K-m of 0.17 +/- 0.03 mu M-1 min(-1). hnpA is necessary for strain CNP-8 to utilize 2C4NP in vivo. HnpC, a BT 1,2-dioxygenase, was proved to catalyze BT ring-cleavage with formation of maleylacetate by HPLC-MS analysis. Phylogenetic analysis indicated that HnpA likely has different evolutionary origin compared to other functionally identified 2C4NP monooxygenases. To our knowledge, this is the first report revealing the catabolic mechanism of 2C4NP via the BT pathway in a Gram-negative bacterium, increasing our knowledge of the catabolic diversity for microbial 2C4NP degradation at the molecular and biochemical level.
WOS关键词	GENE-CLUSTER ; PSEUDOMONAS-STUTZERI ; P-NITROPHENOL ; MONOOXYGENASE CATALYZES ; CATABOLISM PATHWAY ; STRAIN ; BIODEGRADATION ; 4-NITROPHENOL ; 2,4,6-TRICHLOROPHENOL ; IDENTIFICATION
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000483662400030
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; Yantai Science and Technology Project [2017ZH092] ; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24871]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China; 3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Min, J,Xu, LX,Fang, SY,et al. Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(18):7741-7750.
APA	Min, J,Xu, LX,Fang, SY,Chen, WW,&Hu, XK.(2019).Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(18),7741-7750.
MLA	Min, J,et al."Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.18(2019):7741-7750.