Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803

doi:10.1007/s00343-019-7291-1

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803
	Chen Jun ; Shi Wenjun ; Li Wenjun ; Chen Gao ; Qin Song
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2018-11
卷号	36 期号:6 SI 页码:2322-2332
关键词	Synechocystis sp. PCC 6803 genome resequencing non-motile genetic background
ISSN号	2096-5508
DOI	10.1007/s00343-019-7291-1
产权排序	[Chen Jun ; Shi Wenjun ; Li Wenjun ; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Chen Jun ; Shi Wenjun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chen Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
文献子类	Article
英文摘要	Synechocystis sp. PCC 6803 is a model organism widely used in cyanobacterium biology and biotechnology. To know the genetic background of substrains of Synechocystis sp. PCC 6803 is important for further research and application. In this study, we reported the genome sequences of two non-motile wild-type substrains of Synechocystis sp. PCC 6803 using whole genome resequencing. 55/56 putative single nucleotide polymorphisms (SNPs) and 8/9 Indels (insertion and deletion) were identified. Among these, 47 SNPs were found in both the GT-AR and GT-CH strains, and 8 were unique to GT-AR and 9 were unique to GT-CH. All of these variations were annotated in metabolism pathway referred to KEGG database. Meanwhile, the deletion in slr0332 gene was detected in these two strains, which attributed to the non-motile phenotype of them and suggested that the insertion in spkA gene was not essential for non-motile phenotype. These resequencing data provide the genetic background information of these two strains and highlighted the microevolution over decades of laboratory cultivation.
WOS关键词	PURIFICATION ; MOTILITY ; SEQUENCE ; PCC-6803 ; GROWTH
WOS研究方向	Limnology ; Oceanography
语种	英语
WOS记录号	WOS:000454629000034
资助机构	National Key Research and Development Program of China [2016YFF0202304] ; National Natural Science Foundation of China [41376139] ; Science and Technology Program of Yantai City [2016JHZB007] ; Natural Science Foundation of Shandong Province, China [ZR2016CM48]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24570]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen Jun,Shi Wenjun,Li Wenjun,et al. Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6 SI):2322-2332.
APA	Chen Jun,Shi Wenjun,Li Wenjun,Chen Gao,&Qin Song.(2018).Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6 SI),2322-2332.
MLA	Chen Jun,et al."Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6 SI(2018):2322-2332.