烟台市区河流入海口微生物群落的分析 | |
蒲洋; 李文军; 王凯; 赵彦宏; 秦松 | |
刊名 | 海洋科学 |
2017-07-15 | |
卷号 | 41期号:7页码:9-15 |
关键词 | PCR-DGGE Yantai river estuaries PCR-DGGE microorganism diversity analysis 烟台 河流入海口 微生物多样性分析 |
ISSN号 | 1000-3096 |
DOI | DOI: 10.11759/hykx20170112002 |
其他题名 | Analysis of microorganism community in river estuaries of Yantai |
产权排序 | (1) 鲁东大学农学院 ; (2) 中国科学院烟台海岸带研究所 ; (3) 上海海洋大学 |
文献子类 | Article |
英文摘要 | A total of 12 water samples were collected from four river estuaries of Yantai city (Xin, an River, Guangdang River, Yuniao River, and Jiahe River, respectively) in April 2015. The structure of the bacterial community and the dominant groups were examined by PCR-Denatured Gradient Gel Electrophoresis based on 16S rRNA PCR amplification(PCR-DGGE). Results of the analysis demonstrated the presence of high diversities and enrichment in the four river estuaries. Each of the 12 samples showed more than 30 bands, and the Shannon bacterial diversity index varied from 3.3 to 3.61. The Shannon bacterial diversity index was higher in Xinan River and Guangdang River, lower in Yuniao River, and the lowest in Jiahe River. The cluster analysis, by UPGMA analysis, showed that the closer the geographical position was, the higher was the similarity of the bacteria. A total of 14 major DGGE bands were cloned and sequenced. Phylogenetic analysis of the 14 samples showed that Proteobacteria and Bacteroidetes were dominant in the four river estuaries. These results were highly consistent with those of a previous study on the diversity of bacterial communities in the coastal areas of Shandong province. This study could be useful for protecting the environment in the river estuaries of Yantai. |
WOS关键词 | Mineralogy |
WOS研究方向 | Mineralogy ; 矿物学 |
语种 | 中文 |
CSCD记录号 | CSCD:6105498 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/24302] |
专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.鲁东大学农学院; 2.中国科学院烟台海岸带研究所; 3.上海海洋大学 |
推荐引用方式 GB/T 7714 | 蒲洋,李文军,王凯,等. 烟台市区河流入海口微生物群落的分析[J]. 海洋科学,2017,41(7):9-15. |
APA | 蒲洋,李文军,王凯,赵彦宏,&秦松.(2017).烟台市区河流入海口微生物群落的分析.海洋科学,41(7),9-15. |
MLA | 蒲洋,et al."烟台市区河流入海口微生物群落的分析".海洋科学 41.7(2017):9-15. |
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