北黄海沉积物可培养产蛋白酶细菌分离鉴定

doi:DOI: 10.13343/j.cnki.wsxb.20160452

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	北黄海沉积物可培养产蛋白酶细菌分离鉴定
	伍朝亚 ; 李岩 ; 曲慧敏 ; 张振鹏 ; 解志红 ; 孟宪刚
刊名	微生物学报
	2017-04-14
卷号	57 期号:10 页码:1504-1516
关键词	marine sediment protease-producing bacteria diversity serine protease North Yellow Sea 海洋沉积物产蛋白酶细菌多样性丝氨酸蛋白酶北黄海
ISSN号	0001-6209
DOI	DOI: 10.13343/j.cnki.wsxb.20160452
其他题名	Cultivable protease-producing bacteria in the sediments of North Yellow Sea
产权排序	(1) 兰州交通大学化学与生物工程学院 ; (2) 中国科学院烟台海岸带研究所 ; (3) 烟台大学海洋学院
文献子类	Article
英文摘要	[Objective] To uncover the diversity of extracellular protease-producing bacteria and to expand our knowledge on protease-producing bacteria in the sediments of North Yellow Sea, and to screen efficient protease production strains that might provide flora resources for mining marine protease-producing microorganisms. [Methods] Protease-producing bacteria were isolated by using casein gelatin plate from 5 sediment samples of the North Yellow Sea. The bacteria diversity was evaluated through phylogenetic analyses based on 16S rRNA genes. The protease diversity was evaluated through the inhibition tests for 39 strains with higher enzymatic activities by using 4 inhibitors: phenyl methyl sulfonyl fluoride (PMSF, serine protease inhibitor), 1,10-phenanthroline (O-P, metalloproteinase inhibitor), E-64 (cysteine protease inhibitor) and pepstatin A (aspartic protease inhibitor). Furthermore, degradation abilities for different protein substrates such as casein, gelatin and elastin were evaluated through observing hydrolytic zones. [Results] A total of 66 protease-producing strains were isolated from 5 sediment samples. These isolates were classified into 7 genera of 4 phyla including Bacteroidetes, Proteobacteria, Actinobacteria and Firmicutes, with Pseudoalteromonas (69.9%), Sulfitobacter (12.1%) and Salegentibacter (10.6%) as the dominant. The richness of the cultivable protease-producing bacteria reached 10~4 cells/g in all sediment samples. The inhibition tests indicated that all the tested strains produced serine protease and/or metal protease, only a few strains produced cysteine protease or aspartic protease. [Conclusion] The cultivable protease-producing bacteria in the North Yellow Sea are diverse with Pseudoalteromonas, Sulfitobacter and Salegentibacterbacteria as the dominant groups and the extracellular proteases belong to serine proteases and/or metalloproteinases.
WOS关键词	Microbiology
WOS研究方向	Microbiology ; 微生物学
语种	中文
CSCD记录号	CSCD:6077614
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24245]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.兰州交通大学化学与生物工程学院; 2.中国科学院烟台海岸带研究所; 3.烟台大学海洋学院
推荐引用方式 GB/T 7714	伍朝亚,李岩,曲慧敏,等. 北黄海沉积物可培养产蛋白酶细菌分离鉴定[J]. 微生物学报,2017,57(10):1504-1516.
APA	伍朝亚,李岩,曲慧敏,张振鹏,解志红,&孟宪刚.(2017).北黄海沉积物可培养产蛋白酶细菌分离鉴定.微生物学报,57(10),1504-1516.
MLA	伍朝亚,et al."北黄海沉积物可培养产蛋白酶细菌分离鉴定".微生物学报 57.10(2017):1504-1516.