Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571

doi:10.3389/fmicb.2020.585140

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571
	Liu, Xiaolin 1,2; Liu, Yanan 1,2; Johnson, Kevin Scot 3; Dong, Xiaoyan 1,4; Xie, Zhihong 1,4,5
刊名	FRONTIERS IN MICROBIOLOGY
	2020-12-07
卷号	11 页码:12
关键词	chemotaxis CheZ cellular localization Azorhizobium caulinodans rhizobia
ISSN号	1664-302X
DOI	10.3389/fmicb.2020.585140
通讯作者	Xie, Zhihong(zhxie@yic.ac.cn)
英文摘要	Chemotaxis is essential for the competitiveness of motile bacteria in complex and harsh environments. The localization of chemotactic proteins in the cell is critical for coordinating a maximal response to chemotactic signals. One chemotaxis protein with a well-defined subcellular localization is the phosphatase CheZ. CheZ localizes to cell poles by binding with CheA in Escherichia coli and other enteric bacteria, or binding with a poorly understood protein called ChePep in epsilon-Proteobacteria. In alpha-Proteobacteria, CheZ lacks CheA-binding sites, and its cellular localization remains unknown. We therefore determined the localization of CheZ in the alpha-Proteobacteria Azorhizobium caulinodans ORS571. A. caulinodans CheZ, also termed as CheZ(AC,) was found to be located to cell poles independently of CheA, and we suspect that either the N-terminal helix or the four-helix bundle of CheZ(AC) is sufficient to locate to cell poles. We also found a novel motif, AXXFQ, which is adjacent to the phosphatase active motif DXXXQ, which effects the monopolar localization of CheZ(AC). This novel motif consisting of AXXFQ is conserved in CheZ and widely distributed among Proteobacteria. Finally, we found that the substitution of phosphatase active site affects the polar localization of CheZ(AC). In total, this work characterized the localization pattern of CheZ containing a novel motif, and we mapped the regions of CheZ(AC) that are critical for its polar localization.
资助项目	NSFC-Shandong Joint Fund Key Projects[U1806206] ; National Natural Science Foundation of China[31870020]
WOS关键词	CHEMOTAXIS PHOSPHATASE CHEZ ; ESCHERICHIA-COLI ; POLAR LOCALIZATION ; PHOSPHORYLATED CHEY ; SIGNALING PROTEINS ; COMPLEX ; CHEMORECEPTOR ; BACTERIA ; BINDING ; DIVERSITY
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000600397400001
资助机构	NSFC-Shandong Joint Fund Key Projects ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27558]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Xie, Zhihong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 3.Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,et al. Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12.
APA	Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,Dong, Xiaoyan,&Xie, Zhihong.(2020).Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571.FRONTIERS IN MICROBIOLOGY,11,12.
MLA	Liu, Xiaolin,et al."Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571".FRONTIERS IN MICROBIOLOGY 11(2020):12.