The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression

doi:10.1016/j.algal.2021.102214

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression
	Wang, Kang 2; Gao, Zhengquan 2; Wang, Yinchu 1,3; Meng, Chunxiao 2; Li, Jialin 1,3; Qin, Song 1,3; Cui, Yulin 1,3
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2021-04-01
卷号	54 页码:6
关键词	Chlorella vulgaris Chloroplast Genetic engineering Recombinant protein Microparticle bombardment
ISSN号	2211-9264
DOI	10.1016/j.algal.2021.102214
通讯作者	Cui, Yulin(yulincui@yic.ac.cn)
英文摘要	The purpose of this study was to establish a chloroplast transformation system in Chlorella vulgaris for expressing recombinant proteins. The 16S-trnI/trnA-23S in the inverted repeat region of C. vulgaris chloroplast genome was used as the flanking fragments in the homologous recombination vector. Meanwhile, the selectable marker aadA gene was promoted by the endogenous 16S promoter, and two codon-optimized antimicrobial peptide NZ2114 and Piscidin-4 genes linked by endogenous ribosome binding site (RBS) in a polycistron were regulated by the rbcL promoter of C. vulgaris. The plasmid was transformed into the chloroplast of C. vulgaris via microparticle bombardment and the transformants were screened using spectinomycin. After multiple rounds' selection, the homoplastic transformants were identified using polymerase chain reaction (PCR), Southern blotting, and Western blotting. This research provided an efficient chloroplast genetic system for C. vulgaris, and will be benefit for the algal further research and application.
资助项目	National Key Research and Development Program of China[2016YFB0601001] ; National Natural Science Foundation of China[41876188] ; National Natural Science Foundation of China[31972815] ; Natural Science Foundation of Shandong Province, China[ZR2018ZB0210] ; Project of Innovation & Development of Marine Economy[HHCL201803] ; Major Basic Research Program of Shandong Province Natural Science Foundation[ZR2019ZD17] ; Key Research and Development Program of Shandong Province[2018YYSP016]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000632293900009
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province, China ; Project of Innovation & Development of Marine Economy ; Major Basic Research Program of Shandong Province Natural Science Foundation ; Key Research and Development Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27326]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Cui, Yulin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China 2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Gao, Zhengquan,Wang, Yinchu,et al. The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,54:6.
APA	Wang, Kang.,Gao, Zhengquan.,Wang, Yinchu.,Meng, Chunxiao.,Li, Jialin.,...&Cui, Yulin.(2021).The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,54,6.
MLA	Wang, Kang,et al."The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 54(2021):6.