Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions

doi:10.1016/j.fsi.2019.03.009

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions
	Yang, DL ; Han, YJ ; Chen, LZ ; Liu, YL ; Cao, RW ; Wang, Q ; Zhao, JM
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-05
卷号	88 页码:328-334
关键词	Ruditapes philippinarum Scavenger receptor Pattern recognition receptor Immune recognition
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.03.009
产权排序	[Yang, Dinglong ; Han, Yijing ; Liu, Yongliang ; Cao, Ruiwen ; Wang, Qing ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Yang, Dinglong ; Wang, Qing ; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China ; [Han, Yijing ; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
文献子类	Article
英文摘要	In the present study, a scavenger receptor class B type I (designed as RpSR-BI) was cloned and characterized from manila clam Ruditapes philippinarum. The full-length cDNA of RpSR-BI was of 2000 bp, containing an open reading frame (ORF) of 1515 bp. Multiple alignments and phylogenetic analysis strongly suggested that RpSR-BI was a member of the scavenger receptors family. The mRNA transcript of RpSR-BI was constitutively expressed in all tested tissues, and mainly expressed in hepatopancreas and hemocytes. Generally, Vihrio anguillanon or Micrococcus luteus challenge induced the expression of RpSR-BI transcripts in hemocytes of manila clams. Recombinant protein of RpSR-BI (rRpSR-BI) could bind lipopolysaccharides, peptidoglycan and glucan, but not chitin in vitro. Coinciding with the PAMPs binding assay, a broad agglutination spectrum was displayed by rRpSR-BI including Gram-positive bacteria and Gram-negative bacteria. Moreover, rRpSR-BI could enhance the phagocytosis and chemotaxis of hemocytes. These results showed that RpSR-BI functioned as a pattern recognition receptor (PRR) with distinct recognition spectrum, and also as an opsonin involved in the innate immune response of R. philippinarum.
WOS关键词	C-TYPE LECTIN ; PROTEIN ; RECOGNITION ; PEPTIDOGLYCAN ; EXPRESSION ; ADHESION ; CLONING ; ACTS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000465060500039
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Youth Innovation Promotion Association, CAS [2016196]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24947]
专题	海岸带生物资源高效利用研究与发展中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室中国科学院牟平海岸带环境综合试验站
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; 5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Yang, DL,Han, YJ,Chen, LZ,et al. Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:328-334.
APA	Yang, DL.,Han, YJ.,Chen, LZ.,Liu, YL.,Cao, RW.,...&Zhao, JM.(2019).Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions.FISH & SHELLFISH IMMUNOLOGY,88,328-334.
MLA	Yang, DL,et al."Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions".FISH & SHELLFISH IMMUNOLOGY 88(2019):328-334.