A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

doi:10.1039/c9an00243j

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury
	Xu, RF ; Wang, Y ; You, HY ; Zhang, LW ; Wang, YQ ; Chen, LX
刊名	ANALYST
	2019-04-21
卷号	144 期号:8 页码:2556-2564
关键词	IMAGING IN-VITRO SUPEROXIDE ANION XANTHINE-OXIDASE RATIONAL DESIGN LIVER ISCHEMIA FREE-RADICALS MITOCHONDRIA OXYGEN ELECTROCATALYSIS PEROXYNITRITE
ISSN号	0003-2654
DOI	10.1039/c9an00243j
产权排序	[Xu, Runfeng ; You, Huiyan] Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China ; [Xu, Runfeng ; Wang, Yue ; Zhang, Liangwei ; Wang, Yunqing ; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Wang, Yue] Univ Chinese Acad Sci, Beijing 10004, Peoples R China ; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
文献子类	Article
英文摘要	Hydrogen peroxide (H2O2), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2O2. Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2O2. The fluorescence response of the probe is triggered by the reaction of borate esters with H2O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.
WOS关键词	IMAGING IN-VITRO ; SUPEROXIDE ANION ; XANTHINE-OXIDASE ; RATIONAL DESIGN ; LIVER ISCHEMIA ; FREE-RADICALS ; MITOCHONDRIA ; OXYGEN ; ELECTROCATALYSIS ; PEROXYNITRITE
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000467383200008
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21778026, 81573393] ; Program of the Youth Innovation Promotion Association, CAS [2017256, 2019217] ; National Key Scientific Instrument and Equipment Development Projects [2013YQ17052506] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24954]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 2.Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China; 3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 10004, Peoples R China;
推荐引用方式 GB/T 7714	Xu, RF,Wang, Y,You, HY,et al. A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury[J]. ANALYST,2019,144(8):2556-2564.
APA	Xu, RF,Wang, Y,You, HY,Zhang, LW,Wang, YQ,&Chen, LX.(2019).A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury.ANALYST,144(8),2556-2564.
MLA	Xu, RF,et al."A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury".ANALYST 144.8(2019):2556-2564.