Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin

doi:10.1021/acs.analchem.9b00082

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin
	Yang, Q ; Li, JH ; Wang, XY ; Xiong, H ; Chen, LX
刊名	ANALYTICAL CHEMISTRY
	2019-05-21
卷号	91 期号:10 页码:6561-6568
关键词	SENSITIVE DETECTION QUANTUM DOTS CARBON DOTS POLYMERS QDS
ISSN号	0003-2700
DOI	10.1021/acs.analchem.9b00082
产权排序	[Yang, Qian ; Li, Jinhua ; Wang, Xiaoyan ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Yang, Qian ; Xiong, Hua] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China ; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China ; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 13266237, Shandong, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
文献子类	Article
英文摘要	A novel ternary-emission fluorescence sensor was proposed by post-imprinting mixing blue-/green-/red-emission bovine hemoglobin (BHb) imprinted polymers (b-MIPs, g-MIPs, and r-MIPs) at a proper ratio and realized the multiplexed and visual detection of BHb. The three MIPs were individually embedded with blue-emission 7-hydroxycoumarin, green-emission CdTe quantum dots (QDs), and red-emission CdTe/ZnS QDs. Upon interaction with BHb within 8 min, the fluorescence of CdTe and CdTe/ZnS QDs were simultaneously turned off, whereas the 7-hydroxycoumarin turned on the fluorescence intensity. Thereupon, the ratiometric fluorescence intensity of the ternary emission linearly varied within 0.025-3 mu M BHb, accompanying the profuse fluorescence color evolution from yellowish green to yellow to salmon to plum to purple to finally blue. In comparison with the dual- or single-emission sensor, the ternary-emission fluorescence MIPs sensor provided a wider color variation covering the green-red-blue window for accurate naked-eye determination of BHb, as well as a lower detection limit down to 7.8 nM and a higher imprinting factor of 15.2. Moreover, the satisfactory recoveries of 99.25-111.7% in determining the spiked BHb in bovine urine samples, as well as the optical stability and post-imprinting construction convenience, indicated that the developed tricolor-emission fluorescence MIPs sensor provided an ideal alternative for rapid, sensitive, and visual determination of proteins in complicated samples.
WOS关键词	SENSITIVE DETECTION ; QUANTUM DOTS ; CARBON DOTS ; POLYMERS ; QDS
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000469304300023
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21876199, 21575159, 21804010, 21667018, 31860470, 31660482, 41776110]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24938]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 13266237, Shandong, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 4.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Q,Li, JH,Wang, XY,et al. Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin[J]. ANALYTICAL CHEMISTRY,2019,91(10):6561-6568.
APA	Yang, Q,Li, JH,Wang, XY,Xiong, H,&Chen, LX.(2019).Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin.ANALYTICAL CHEMISTRY,91(10),6561-6568.
MLA	Yang, Q,et al."Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin".ANALYTICAL CHEMISTRY 91.10(2019):6561-6568.