Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay

doi:10.1039/c9nr04864b

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay
	Fu, XL ; Wen, JH ; Li, JW ; Lin, H ; Liu, YM ; Zhuang, XM ; Tian, CY ; Chen, LX
刊名	NANOSCALE
	2019-09-07
卷号	11 期号:33 页码:15530-15536
关键词	POLYMERASE-CHAIN-REACTION GOLD NANOPARTICLES STRIP BIOSENSOR PCR AMPLIFICATION QUANTIFICATION IMMUNOASSAY SUBSTRATE STRATEGY BINDING
ISSN号	2040-3364
DOI	10.1039/c9nr04864b
产权排序	[Fu, Xiuli ; Wen, Jiahui ; Li, Jingwen ; Lin, Hao ; Liu, Yongming ; Zhuang, Xuming ; Tian, Chunyuan] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China ; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
文献子类	Article
英文摘要	Accurate analysis of prostate cancer specific biomarkers plays an important role in the early diagnosis of prostate cancer. Traditional colorimetric lateral flow assay (LFA) has the limitations of low detection sensitivity and qualitative or semiquantitative detection. In this study, we developed a novel surface-enhanced Raman scattering (SERS)-based competitive LFA for the rapid and highly sensitive quantitative evaluation of prostate cancer antigen 3 (PCA3) mimic DNA. Herein, the competitive hybridization interaction with capture DNA between target PCA3 mimic DNA and reporter DNA-labeled SERS nanotags results in a change in the amount of SERS nanotags on the test line. The quantitative analysis of target PCA3 mimic DNA was realized by monitoring the Raman peak intensity of SERS nanotags on the test line. The limit of detection of PCA3 mimic DNA was estimated to be 3 fM, which is about three orders of magnitude more sensitive than that of a commercially available kit. By combining the outstanding characteristics of the well-established SERS-based competitive strategy and LFA platform, our design has strong potential for the early diagnosis of prostate cancer and other diseases.
WOS关键词	POLYMERASE-CHAIN-REACTION ; GOLD NANOPARTICLES ; STRIP BIOSENSOR ; PCR ; AMPLIFICATION ; QUANTIFICATION ; IMMUNOASSAY ; SUBSTRATE ; STRATEGY ; BINDING
WOS研究方向	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
语种	英语
WOS记录号	WOS:000484345500008
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21575159, 21778047] ; National Natural Science Foundation of Shandong Province in China [ZR2017BB026]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24860]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; 3.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China; 4.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China;
推荐引用方式 GB/T 7714	Fu, XL,Wen, JH,Li, JW,et al. Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay[J]. NANOSCALE,2019,11(33):15530-15536.
APA	Fu, XL.,Wen, JH.,Li, JW.,Lin, H.,Liu, YM.,...&Chen, LX.(2019).Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay.NANOSCALE,11(33),15530-15536.
MLA	Fu, XL,et al."Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay".NANOSCALE 11.33(2019):15530-15536.