The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device

doi:10.1016/j.bios.2019.111533

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device
	Qi, J ; Li, BW ; Zhou, N ; Wang, XY ; Deng, DM ; Luo, LQ ; Chen, LX
刊名	BIOSENSORS & BIOELECTRONICS
	2019-10-01
卷号	142 页码:UNSP 111533
关键词	Paper based microfluidic device Bioinspired molecularly imprinted technique Electrochemical detection Valve Point of care diagnosis Cancer markers
ISSN号	0956-5663
DOI	10.1016/j.bios.2019.111533
产权排序	[Qi, Ji ; Deng, Dongmei ; Luo, Liqiang] Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China ; [Qi, Ji ; Li, Bowei ; Zhou, Na ; Wang, Xiaoyan ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China ; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	In this work, we provided a novel strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers (MIPs) on movable valve microfluidic paper-based electrochemical device (Bio-MIP-ePADs) for clinical detection of biomarkers. The newly movable valves on the device enable continuous and convenient delivery of fluid, which guarantee the performance for fabricating MIPs structure during long time electro-polymerization. Moreover, this strategy can directly detect antigens by taking advantage of molecular imprinting on paper-based device, which greatly decreases the cost during clinical testing, reduces the tedious washing procedure and does not need to consider the preservation of the antibody in enzyme linked immunosorbent assay (ELISA). This feature makes the chip suitable for the on-site family treatment or commercial products. To further validate the applicability of this proposed method for clinical diagnostic testing, carcinoembryonic antigen (CEA) was applied as prototyping model target for the clinical analysis. The proposed Bio-MIP-ePADs were cheap, easy to prepare, disposable and provided reliable analysis by comparing with ELISA. We hope the application of this technology will open up a new avenue to the point-of-care testing (POCT).
WOS关键词	ELECTROCHEMILUMINESCENCE ; PLATFORM ; MICROFLUIDICS ; CHALLENGES ; SENSORS ; ASSAY
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
语种	英语
WOS记录号	WOS:000487175000057
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 61571278, 61571280, 21804010] ; National Key Research and Development Program of China [2016YFC1400702] ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24849]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; 2.Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Qi, J,Li, BW,Zhou, N,et al. The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device[J]. BIOSENSORS & BIOELECTRONICS,2019,142:UNSP 111533.
APA	Qi, J.,Li, BW.,Zhou, N.,Wang, XY.,Deng, DM.,...&Chen, LX.(2019).The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device.BIOSENSORS & BIOELECTRONICS,142,UNSP 111533.
MLA	Qi, J,et al."The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device".BIOSENSORS & BIOELECTRONICS 142(2019):UNSP 111533.