Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays

doi:10.1021/acs.analchem.9b04522

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays
	Wu, Y ; Choi, N ; Chen, H ; Dang, H ; Chen, LX ; Choo, J
刊名	ANALYTICAL CHEMISTRY
	2020-02-04
卷号	92 期号:3 页码:2628-2634
关键词	MULTIPLEX DETECTION AMPLIFICATION-FREE SERS PCR DNA SPECTROSCOPY IMMUNOASSAY MUTATIONS DIAGNOSIS
ISSN号	0003-2700
DOI	10.1021/acs.analchem.9b04522
产权排序	[Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Wu, Yixuan ; Chen, Hao ; Dang, Hajun ; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea ; [Choi, Namhyun] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea
文献子类	Article
英文摘要	We report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene, but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform and evaluated its performance by sequentially measuring the Raman signals of B. anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limits of detection (LODs) than RT-qPCR under the small cycle number of 20. Particularly, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15, but SERS-PCR enabled DNA detection after only five cycles with an LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1-1000 pM) is wider than that for RTqPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future.
WOS关键词	MULTIPLEX DETECTION ; AMPLIFICATION-FREE ; SERS ; PCR ; DNA ; SPECTROSCOPY ; IMMUNOASSAY ; MUTATIONS ; DIAGNOSIS
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000511509400038
资助机构	National Research Foundation of KoreaNational Research Foundation of Korea [2019R1A2C3004375] ; Chung-Ang University
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24755]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; 3.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea
推荐引用方式 GB/T 7714	Wu, Y,Choi, N,Chen, H,et al. Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays[J]. ANALYTICAL CHEMISTRY,2020,92(3):2628-2634.
APA	Wu, Y,Choi, N,Chen, H,Dang, H,Chen, LX,&Choo, J.(2020).Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays.ANALYTICAL CHEMISTRY,92(3),2628-2634.
MLA	Wu, Y,et al."Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays".ANALYTICAL CHEMISTRY 92.3(2020):2628-2634.