Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe

doi:10.1021/acs.analchem.8b01442

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe
	Wang, Yue ; Gao, Min ; Chen, Qingguo ; Yu, Fabiao ; Jiang, Guibin ; Chen, Lingxin
刊名	ANALYTICAL CHEMISTRY
	2018-08-21
卷号	90 期号:16 页码:9769-9778
关键词	REACTIVE OXYGEN LIVE CELLS ENDOGENOUS SUPEROXIDE HYDROGEN POLYSULFIDES INORGANIC MERCURY AQUEOUS-SOLUTION ENERGY-TRANSFER LIVING CELLS IN-VITRO ON PROBE
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b01442
产权排序	[Wang, Yue ; Gao, Min ; Yu, Fabiao ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Jiang, Guibin] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China ; [Chen, Qingguo ; Yu, Fabiao ; Chen, Lingxin] Zhejiang Ocean Univ, Sch Marine Sci & Technol, Zhoushan 316022, Peoples R China ; [Wang, Yue ; Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	As a cytotoxic heavy metal ion, mercury(II) ion (Hg2+) induces severe oxidative stress and further results in physiological dysfunction. Although mercury poisoning can be treated with many drugs, such as sodium selenite, the therapeutic effect is relatively poor, and it seems that the damage to human health continues. However, the interpretation for the pathogenesis has not been clarified yet. We supposed that the reason is attributed to Hg2+-caused intracellular oxidative stress. To confirm our hypothesis, we strived to design a three-channel ratio fluorescent probe, HCy-SeH, for superoxide anion (O-2(center dot-)) and Hg2+ combined detection. O-2(center dot-) is a vital precursor for other reactive oxygen species (ROS), which is involved in many physiological and pathological processes. However, until now there is no efficient chemical tool for O-2(center dot-) and Hg2+ combined detection in cells and in vivo. The fluorescence response of our probe is initiated by a hydrogen abstraction reaction from the hydrocyanine fluorophore moiety. Once oxidized by O-2(center dot-), HCy-SeH recovers its pi-conjugated system back to a heptamethine cyanine derivative, Cy-SeH. Cy-SeH coexists with its conjugate base,Cy=Se. One emits red fluorescence, and the other one emits green fluorescence. The response unit, -SeH, can trap Hg2+ via a Se-Hg antagonism reaction to afford an orange-emitting final product, Keto-Cy. The probe offers high selectivity and sensitivity toward O-2(center dot-). and Hg2+. When applied for O-2(center dot-) and Hg2+ detection in HEK 293 cells, the imaging results indicate that our probe can provide a combined response for O-2(center dot-). and Hg2+ in real time and in situ. Flow cytometry analysis is well-consistent with the results from fluorescence imaging. When applied to image O-2(center dot-) and Hg2+ in mice models, we find that Hg2+ dominantly accumulates in the kidney and induces a burst of O-2(center dot-). We confirm that chronic mercurialism can cause severe oxidative damage and renal fibrosis. HCy-SeH further provides a new information that, even when intracellular Hg2+ has been antagonized, the outbreak of O-2(center dot-). caused by mercury poisoning still lasts.
WOS关键词	REACTIVE OXYGEN ; LIVE CELLS ; ENDOGENOUS SUPEROXIDE ; HYDROGEN POLYSULFIDES ; INORGANIC MERCURY ; AQUEOUS-SOLUTION ; ENERGY-TRANSFER ; LIVING CELLS ; IN-VITRO ; ON PROBE
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000442707100018
资助机构	National Nature Science Foundation of China [21575159, 21775162, 41776110, 41506126, 21405172] ; program of Youth Innovation Promotion Association, CAS [2015170] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662] ; Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology [SATM201705]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24516]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; 3.Zhejiang Ocean Univ, Sch Marine Sci & Technol, Zhoushan 316022, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yue,Gao, Min,Chen, Qingguo,et al. Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2018,90(16):9769-9778.
APA	Wang, Yue,Gao, Min,Chen, Qingguo,Yu, Fabiao,Jiang, Guibin,&Chen, Lingxin.(2018).Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe.ANALYTICAL CHEMISTRY,90(16),9769-9778.
MLA	Wang, Yue,et al."Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe".ANALYTICAL CHEMISTRY 90.16(2018):9769-9778.