Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater

doi:10.1039/c8an00811f

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater
	Li, Jinhua ; Fu, Junqing ; Yang, Qian ; Wang, Liyan ; Wang, Xiaoyan ; Chen, Lingxin
刊名	ANALYST
	2018-08-07
卷号	143 期号:15 页码:3570-3578
关键词	TURN-ON POLYMER NANOPARTICLES MICROSPHERES
ISSN号	0003-2654
DOI	10.1039/c8an00811f
产权排序	[Li, Jinhua ; Yang, Qian ; Wang, Liyan ; Wang, Xiaoyan ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Fu, Junqing] Prov Food & Drug Inspect Inst Shandong, Jinan 250101, Shandong, Peoples R China ; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China ; [Chen, Lingxin] Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China ; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
文献子类	Article
英文摘要	Versatile molecular imprinted fluorescence sensors have been prepared for various species, but the imprinting based fluorescence detection of target proteins upon different external stimulation is rarely reported. Herein, a novel phycocyanin-imprinted ratiometric fluorescence nanosensor was developed for the temperature regulated sensing and detection of a phycocyanin target based on fluorescence resonance energy transfer (FRET). The nanosensor was fabricated via simple facile copolymerization, with amino/carboxyl modified quantum dots (QDs) as a fluorescent support, N-isopropylacrylamide as a thermo-responsive functional monomer, N,N-methylenebisacrylamide as a cross-linker and phycocyanin as a template. Under temperature control at 20 degrees C and 45 degrees C, the fluorescence intensities of the QDs and phycocyanin were regularly decreased and enhanced, respectively, to a different extent as the concentration of phycocyanin increased, and thereby the ratio of the two fluorescence peak emission intensities of QDs and phycocyanin was used to determine the concentration of phycocyanin. Good linearity was obtained within the range of 0-1.8 M (r = 0.9900) with a low detection limit of 3.2 nM, and excellent recognition selectivity towards the phycocyanin target was achieved over other proteins. Moreover, satisfactory recoveries of 92.0-106.8% were obtained in spiked seawater samples. This study provides a facile, fast and intelligent way to conduct identification analysis of trace proteins in complex water matrices, and can push forward protein imprinting and stimuli-responsive imprinting related research.
WOS关键词	TURN-ON ; POLYMER ; NANOPARTICLES ; MICROSPHERES
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000439586400008
资助机构	National Natural Science Foundation of China [21477160, 41776110] ; Natural Science Foundation of Shandong Province of China [ZR2016BL25, ZR2016DB07] ; National Defence Science and Technology Innovation Project of the Chinese Academy of Sciences [CXJJ-16M254] ; Department of Science and Technology of Shandong Province of China [GG201709290055]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24426]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Prov Food & Drug Inspect Inst Shandong, Jinan 250101, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 4.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China; 5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Li, Jinhua,Fu, Junqing,Yang, Qian,et al. Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater[J]. ANALYST,2018,143(15):3570-3578.
APA	Li, Jinhua,Fu, Junqing,Yang, Qian,Wang, Liyan,Wang, Xiaoyan,&Chen, Lingxin.(2018).Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater.ANALYST,143(15),3570-3578.
MLA	Li, Jinhua,et al."Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater".ANALYST 143.15(2018):3570-3578.