Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin

doi:10.1021/acssensors.7b00804

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin
	Wang, Xiaoyan ; Yu, Shuangmei ; Liu, Wen ; Fu, Longwen ; Wang, Yunqing ; Li, Jinhua ; Chen, Lingxin
刊名	ACS SENSORS
	2018-02
卷号	3 期号:2 页码:378-385
关键词	molecular imprinting protein imprinting ratiometric fluorescence bovine hemoglobin quantum dots
ISSN号	2379-3694
DOI	10.1021/acssensors.7b00804
产权排序	[Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China ; [Wang, Xiaoyan ; Liu, Wen ; Fu, Longwen ; Wang, Yunqing ; Li, Jinhua ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Yu, Shuangmei] Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
文献子类	Article
英文摘要	We describe a simple and effective strategy to construct a molecular imprinting ratiometric fluorescence sensor (MIR sensor) for the visual detection of bovine hemoglobin (BHb) used as a model protein. The sensor was prepared by simply mixing the solution of green and red CdTe quantum dots (QDs), which were embedded in core-shell structured molecularly imprinted polymers and silica nano-particles, respectively. The resultant hybrid MIR sensor can selectively bind with BHb and thus quench the fluorescence of the green QDs, while the red QDs wrapped with silica are insensitive to BHb with the fluorescence intensity unchanged. As a result, a continuous obvious fluorescence color change from green to red can be observed by naked eyes, with the detection limit of 9.6 nM. Moreover, the MIR sensor was successfully applied to determine BHb in bovine urine samples with satisfactory recoveries at three spiking levels ranging from 95.7 to 101.5%, indicating great potential application for detecting BHb in real samples. This strategy of using different fluorescence emission materials incorporated to construct a ratiometric fluorescence sensor is reasonable and convenient, which can be extended to the preparation of other ratiometric fluorescence systems for targeted analytes.
WOS关键词	CDTE QUANTUM DOTS ; DOPED ZNS QDS ; TARGET PROTEIN ; SENSITIVE DETECTION ; ALPHA-FETOPROTEIN ; SERUM-ALBUMIN ; TURN-ON ; POLYMER ; RECOGNITION ; SURFACE
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000426618400017
资助机构	National Natural Science Foundation of China [21477160, 21575159, 81573393] ; Science and Technology Development Plan of Shandong Province of China [2014GGF01074] ; Youth Innovation Promotion Association CAS [2017256] ; Defense Science and Technology Innovation Fund of the Chinese Academy of Sciences [CXJJ-17-Q148] ; Instrument Developing Project of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24397]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Xiaoyan,Yu, Shuangmei,Liu, Wen,et al. Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin[J]. ACS SENSORS,2018,3(2):378-385.
APA	Wang, Xiaoyan.,Yu, Shuangmei.,Liu, Wen.,Fu, Longwen.,Wang, Yunqing.,...&Chen, Lingxin.(2018).Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin.ACS SENSORS,3(2),378-385.
MLA	Wang, Xiaoyan,et al."Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin".ACS SENSORS 3.2(2018):378-385.