A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress

doi:10.1016/j.biomaterials.2017.04.024

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress
	Dou, K ; Fu, Q ; Chen, G ; Yu, FB ; Liu, YX ; Cao, ZP ; Li, GL ; Zhao, XN ; Xia, L ; Chen, LX
刊名	BIOMATERIALS
	2017-07
卷号	133 页码:82-93
关键词	Dual-response Dual-ratiometric Quantification SO2/ClO- Imaging analysis
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2017.04.024
产权排序	[Dou, Kun ; Fu, Qiang ; Chen, Guang ; Liu, Yuxia ; Cao, Ziping ; Li, Guoliang ; Zhao, Xianen ; Xia, Lian ; Wang, Hua ; You, Jinmao] Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China ; [Chen, Guang] Shandong Normal Univ, Engn Res Ctr Pesticide & Med Intermediate Clean P, Chem Engn & Mat Sci, Coll Chem,Minist Educ, Jinan 250014, Peoples R China ; [Yu, Fabiao ; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remedia, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO2 can be generated as the anti-oxidant, while SO2 is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO2 under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO2 and ClO-. The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO2 and ClO-, allowing the highly selective and accurate ratiometric quantification for both SO2 and ClO- in cells. Moreover the ultrafast (SO2: <60 s; ClO-: within sec) and highly sensitive (detection limits: SO2: 3.5 nM; ClO-: 12.5 nM) detection were achieved. With the robust applicability, the developed probe was successfully used to quantify SO2 and endogenous ClO- in respectively the HeLa cells and the RAW 264.7 cells, as well as to visualize the dynamic of SO2/ClO- in zebrafish. The fluorescent imaging studies and flow cytometry analysis confirmed the burst-and-depletion and meanwhile the oxidative-and-antioxidative effects of intracellular SO2 under the NaClO induced oxidative stress. (C) 2017 Elsevier Ltd. All rights reserved.
WOS关键词	Dual-response ; Dual-ratiometric ; Quantification ; SO2/ClO- ; Imaging analysis
WOS研究方向	Engineering ; Materials Science
语种	英语
WOS记录号	WOS:000401716700009
资助机构	National Natural Science Foundation of China [21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076] ; Shandong Province Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-6] ; Opening Foundation of Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-9] ; Taishan Scholar Foundation of Shandong Province, PR China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24362]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remedia, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China; 3.Shandong Normal Univ, Engn Res Ctr Pesticide & Med Intermediate Clean P, Chem Engn & Mat Sci, Coll Chem,Minist Educ, Jinan 250014, Peoples R China;
推荐引用方式 GB/T 7714	Dou, K,Fu, Q,Chen, G,et al. A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress[J]. BIOMATERIALS,2017,133:82-93.
APA	Dou, K.,Fu, Q.,Chen, G.,Yu, FB.,Liu, YX.,...&You, JM.(2017).A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress.BIOMATERIALS,133,82-93.
MLA	Dou, K,et al."A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress".BIOMATERIALS 133(2017):82-93.