Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations

doi:10.1021/acs.analchem.8b05971

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	Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations
	Liu, SW ; Ding, JW ; Qin, W
刊名	ANALYTICAL CHEMISTRY
	2019-02-19
卷号	91 期号:4 页码:3170-3176
关键词	ION-SELECTIVE ELECTRODES SOLID-CONTACT LABEL-FREE COLORIMETRIC DETECTION MEMBRANE ELECTRODES APTAMER MOLECULES CYSTEINE SYSTEMS SENSORS
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b05971
产权排序	[Liu, Shuwen ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China ; [Liu, Shuwen ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China ; [Ding, Jiawang ; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China ; [Liu, Shuwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Conventional potentiometric ion sensors that rely on a specific ion carrier in a polymeric membrane can hardly achieve multianalyte detection. Inspired by the remarkable ability of built-in logic gate sensors for multianalyte detection, herein we report a potentiometric aptasensing platform based on a G-quadruplex/hemin DNAzyme and logic gate operations for determination of two analytes using a single membrane electrode. A bifunctional probe with two aptamer units and a signal reporter oligonucleotide with a DNAzyme sequence are assembled on the magnetic beads to form a DNA hybrid structure. The "OR" and "INHIBIT" logic functions can be performed by using the two aptamers and their targets as inputs, and using the chronopotentiometric response based on the G-quadruplex/hemin DNAzyme-H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine as output. Kanamycin and oxytetracycline, as commonly used antibiotics, have been employed as the models and successfully measured.
WOS关键词	ION-SELECTIVE ELECTRODES ; SOLID-CONTACT ; LABEL-FREE ; COLORIMETRIC DETECTION ; MEMBRANE ELECTRODES ; APTAMER ; MOLECULES ; CYSTEINE ; SYSTEMS ; SENSORS
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000459642400083
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21575158, 21475148, 41876108] ; National Key Research and Development Program of China [2016YFC1400700] ; Instrument Developing Project of the Chinese Academy of Sciences [Y728021021] ; Taishan Scholar Program of Shandong Province [TS20081159]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24998]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Liu, SW,Ding, JW,Qin, W. Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations[J]. ANALYTICAL CHEMISTRY,2019,91(4):3170-3176.
APA	Liu, SW,Ding, JW,&Qin, W.(2019).Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations.ANALYTICAL CHEMISTRY,91(4),3170-3176.
MLA	Liu, SW,et al."Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations".ANALYTICAL CHEMISTRY 91.4(2019):3170-3176.