Liver transcriptome analysis reveals the molecular responses to low-salinity in large yellow croaker Larimichthys crocea

doi:10.1016/j.aquaculture.2019.734827

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Liver transcriptome analysis reveals the molecular responses to low-salinity in large yellow croaker Larimichthys crocea
	Lu, Z ; Huang, WQ ; Wang, S ; Shan, XJ ; Ji, CL ; Wu, HF
刊名	AQUACULTURE
	2020-02-25
卷号	517 页码:UNSP 734827
关键词	Larimichthys crocea Transcriptome Low salinity Molecular response
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2019.734827
产权排序	[Lu, Zhen ; Wang, Shuang ; Ji, Chenglong ; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Lu, Zhen ; Wang, Shuang ; Ji, Chenglong ; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China ; [Lu, Zhen ; Shan, Xiujuan ; Ji, Chenglong ; Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China ; [Lu, Zhen ; Wang, Shuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Huang, Weiqing] Ningde Dingcheng Fisheries Co Ltd Fujian, Ningde 352100, Peoples R China ; [Huang, Weiqing] Ningde Normal Univ, Dept Bioengn, Ningde 352100, Peoples R China ; [Ji, Chenglong ; Wu, Huifeng] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
文献子类	Article
英文摘要	The large yellow croaker Larimichthys crocea is a euryhaline marine fish with wide consumptions and high commercial values in China. Low-salinity culture of L. crocea has been proposed to suppress the pathogens with low cost. However, the responses of L. crocea at molecular level to low salinity have not been fully understood. In this study, Illumina RNA-seq gene expression system was applied to globally screen the genes and pathways involved in the responses in liver tissues of L. crocea to low-salinity conditions. Compared with the control (24 ppt), a total of 1369, 685, 2980 and 3469 differentially expressed genes (DEGs) were identified in low-salinity treatments (8, 6, 4 and 2 ppt) for 40 days, respectively. Among them, 164 DEGs were common in 4 low-salinity groups. According to the KEGG pathway analysis, the DEGs were involved in material metabolism (carbohydrate, protein, and lipid metabolism), energy metabolism, growth, immune system, signal transduction, transcription and translation, and apoptosis. With respect to metabolism, up-regulated oxidative phosphorylation was the primary enriched pathways in response to low salinities of 6 ppt (S6) and 4 ppt (S4). The salinities of 8 ppt (S8) and 2 ppt (S2) were characterized by enhanced anabolism and inhibited material and energy metabolism, respectively. Concerning immune responses, innate immunity was promoted in S6 and S4 groups as a general stress response. L. crocea cultured in S4 group suffered few immune and inflammation risks, indicated by the negative responses to CD209 antigen (CD209) and C-reactive protein (crp). Moreover, low salinities induced higher absolute weight gain rate (AWGR) and survival rate of L. crocea, and S4 group presented the highest AWGR and survival rate. Our findings indicated that salinity of 4 ppt was preferable for the low-salinity farming of L. crocea. Overall, this work provides new insights into the molecular responses of L. crocea to low-salinity and references for L. crocea culture in low salinities.
WOS关键词	BASS DICENTRARCHUS-LABRAX ; DC-SIGN ; GROWTH ; EXPRESSION ; OSMOREGULATION ; TEMPERATURE ; ADAPTATION ; STRESS ; METABOLISM ; CONVERSION
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000509541400025
资助机构	Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018-MFS-T02] ; Supporting Project of Fujian Province for "Science and Technology Service Network Initiative, CAS" [2017T3016] ; Young Taishan Scholars Program of Shandong Province [tsqn201812115] ; Ningde Normal Univeristy Special Fund Program [2019ZX406] ; Research Center for Targeted Poverty Alleviation and Anti-return to Poverty
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24746]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; 2.Ningde Dingcheng Fisheries Co Ltd Fujian, Ningde 352100, Peoples R China; 3.Ningde Normal Univ, Dept Bioengn, Ningde 352100, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Lu, Z,Huang, WQ,Wang, S,et al. Liver transcriptome analysis reveals the molecular responses to low-salinity in large yellow croaker Larimichthys crocea[J]. AQUACULTURE,2020,517:UNSP 734827.
APA	Lu, Z,Huang, WQ,Wang, S,Shan, XJ,Ji, CL,&Wu, HF.(2020).Liver transcriptome analysis reveals the molecular responses to low-salinity in large yellow croaker Larimichthys crocea.AQUACULTURE,517,UNSP 734827.
MLA	Lu, Z,et al."Liver transcriptome analysis reveals the molecular responses to low-salinity in large yellow croaker Larimichthys crocea".AQUACULTURE 517(2020):UNSP 734827.