Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode

doi:10.1021/acs.analchem.7b03522

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode
	Ding, Jiawang ; Yu, Nana ; Wang, Xuedong ; Qin, Wei
刊名	ANALYTICAL CHEMISTRY
	2018-02-06
卷号	90 期号:3 页码:1734-1739
关键词	PULSED GALVANOSTATIC CONTROL POLYMERIC MEMBRANE ELECTROCHEMICAL DETECTION LIQUID-MEMBRANE LABEL-FREE SENSORS SUBSTRATE PRINCIPLE PROTAMINE HEPARIN
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b03522
产权排序	[Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China ; [Ding, Jiawang ; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China ; [Yu, Nana ; Wang, Xuedong] Wenzhou Med Univ, Key Lab Watershed Sci & Hlth Zhejiang Prov, Dept Environm Sci, Wenzhou 325035, Peoples R China
文献子类	Article
英文摘要	A polymeric membrane ion-selective electrode (ISE) is typically designed for the determination of one specific ion using a conventional method. In this work, we demonstrate a simple, versatile, and sensitive platform for simultaneous detection of two molecules with a single ISE. Under a series of periodic galvanostatic polarization, a solid-contact ISE without ion exchanger properties under zero-current conditions has been successfully used for simultaneous detection of two opposite charged ions with high sensitivity, good selectivity, and fast reversibility. By integration of biorecognition elements with the potentiometric measurement, highly sensitive and selective detection of a broad range of different molecular targets can be predicted. As a proof of concept, a potentiometric genosensor based on magnetic beads-enzyme sandwich assay has been designed for sensitive and selective detection of pathogenic bacteria Escherichia coli O157:H7 and Staphylococcus aureus. Under optimal conditions, two bacteria nucleic acid sequences can be detected simultaneously with high sensitivity and good selectivity by using a single solid-contact potentiometric ISE. The detection limits of Escherichia coli O157:H7 DNA and Staphylococcus aureus DNA are 120 and 54 fM (3 sigma), respectively. Because of its simplicity, this potentiometric technique based on ISE can be an attractive tool or detector to perform two analyte measurements.
WOS关键词	PULSED GALVANOSTATIC CONTROL ; POLYMERIC MEMBRANE ; ELECTROCHEMICAL DETECTION ; LIQUID-MEMBRANE ; LABEL-FREE ; SENSORS ; SUBSTRATE ; PRINCIPLE ; PROTAMINE ; HEPARIN
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000424730600043
资助机构	National Natural Science Foundation of China [21575158, 21677172] ; Instrument Developing Project of the Chinese Academy of Sciences [Y728021021] ; National Key Research and Development Program of China [2016YFC1400700] ; Taishan Scholar Program of Shandong Province [TS20081159]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24396]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; 2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; 3.Wenzhou Med Univ, Key Lab Watershed Sci & Hlth Zhejiang Prov, Dept Environm Sci, Wenzhou 325035, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jiawang,Yu, Nana,Wang, Xuedong,et al. Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode[J]. ANALYTICAL CHEMISTRY,2018,90(3):1734-1739.
APA	Ding, Jiawang,Yu, Nana,Wang, Xuedong,&Qin, Wei.(2018).Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode.ANALYTICAL CHEMISTRY,90(3),1734-1739.
MLA	Ding, Jiawang,et al."Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode".ANALYTICAL CHEMISTRY 90.3(2018):1734-1739.