Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process

doi:10.1016/j.jes.2020.07.025

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process
	Sun, Yanyu 1,2,3,4; Chen, Weiwei 1,2,3,4; Wang, Yibo 1,2,3,4; Guo, Jie 4; Zhang, Haikun 2,3,4; Hu, Xiaoke 2,3,4
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2021-02-01
卷号	100 页码:317-327
关键词	Crude oil bioaugmentation Bacterial community dynamic shift Enhanced green fluorescent protein
ISSN号	1001-0742
DOI	10.1016/j.jes.2020.07.025
通讯作者	Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要	The biodegradation was considered as the prime mechanism of crude oil degradation. To validate the efficacy and survival of the crude oil-degrading strain in a bioremediation process, the enhanced green fluorescent protein gene (egfp) was introduced into Acinetobacter sp. HC8-3S. In this study, an oil-contaminated sediment microcosm was conducted to investigate the temporal dynamics of the physicochemical characterization and microbial community in response to bacterium amendment. The introduced strains were able to survive, flourish and degrade crude oil quickly in the early stage of the bioremediation. However, the high abundance cannot be maintained due to the ammonium (NH4+-N) and phosphorus (PO43--P) contents decreased rapidly after 15 days of remediation. The sediment microbial community changed considerably and reached relatively stable after nutrient depletion. Therefore, the addition of crude oil and degrading cells did not show a long-time impact on the original microbial communities, and sufficient nitrogen and phosphorus nutrients ensures the survive and activity of degrader. Our studies expand the understanding of the crude oil degradative processes, which will help to develop more rational bioremediation strategies. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
资助项目	International Cooperation, Chinese Academy of Sciences, Chinese-foreign Cooperation in key projects The detection of oil spill and its ecological impact study[133337KYSB20160002] ; State's Key Project of Research and Development Plan[2016YFC1402300]
WOS关键词	CONTAMINATED SOIL ; DEGRADATION ; COMMUNITY ; STRAIN ; BIOREMEDIATION ; IDENTIFICATION ; BIODEGRADATION ; BIOSTIMULATION ; BACTERIA ; REFINERY
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000596939800032
资助机构	International Cooperation, Chinese Academy of Sciences, Chinese-foreign Cooperation in key projects The detection of oil spill and its ecological impact study ; State's Key Project of Research and Development Plan
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27525]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Hu, Xiaoke
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yanyu,Chen, Weiwei,Wang, Yibo,et al. Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2021,100:317-327.
APA	Sun, Yanyu,Chen, Weiwei,Wang, Yibo,Guo, Jie,Zhang, Haikun,&Hu, Xiaoke.(2021).Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process.JOURNAL OF ENVIRONMENTAL SCIENCES,100,317-327.
MLA	Sun, Yanyu,et al."Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process".JOURNAL OF ENVIRONMENTAL SCIENCES 100(2021):317-327.