Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum

doi:10.1016/j.envpol.2021.116443

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum
	Zhan, Junfei 1,2,3; Wang, Shuang 1,2,3; Li, Fei 2,3,5; Ji, Chenglong 2,3,4,5; Wu, Huifeng 2,3,4,5
刊名	ENVIRONMENTAL POLLUTION
	2021-03-15
卷号	273 页码:9
关键词	Benchmark dose Cadmium Dose-dependent effects Ruditapes philippinarum Transcriptomics
ISSN号	0269-7491
DOI	10.1016/j.envpol.2021.116443
通讯作者	Wu, Huifeng(hfwu@yic.ac.cn)
英文摘要	Cadmium (Cd) is being frequently detected in marine organisms. However, dose-dependent effects of Cd challenged unraveling the toxicological mechanisms of Cd to marine organisms and developing biomarkers. Here, the dose-dependent effects of Cd on clams Ruditapes philippinarum following exposure to 5 doses of Cd (3, 9, 27, 81, 243 mg/L) were investigated using benchmark dose (BMD) method. By model fitting, calculation of BMD values was performed on transcriptomic profiles, metals concentrations, and antioxidant indices. Cd exposure induced not only significant Cd accumulation in clams, but also marked alterations of essential metals such as Ca, Cu, Zn, Mn, and Fe. Gene regulation posed little influence on essential metal homeostasis, indicated by poor enrichment of differentially expressed genes (DEGs) associated with metal binding and metal transport in lower concentrations of Cd-treated groups. BMD analysis on biological processes and pathways showed that peptide cross-linking was the most sensitive biological process to Cd exposure, followed by focal adhesion, ubiquitin mediated proteolysis, and apoptosis. Occurrence of apoptosis was also confirmed by TUENL-positive staining in gills and hepatopancreas of clams treated with Cd. Furthermore, many DEGs, such as transglutaminases (TGs), metallothionein (MT), STEAP2-like and laccase, which presented linear or monotonic curves and relatively low BMD values, were potentially preferable biomarkers in clams to Cd. Overall, BMD analysis on transcriptomic profiles, metals concentrations and biochemical endpoints unraveled the sensitiveness of key events in response to Cd treatments, which provided new insights in exploring the toxicological mechanisms of Cd in clams as well as biomarker selection. (C) 2021 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[42076164] ; National Natural Science Foundation of China[41676114] ; Young Taishan Scholars Program of Shandong Province[tsqn201812115]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000625376600033
资助机构	National Natural Science Foundation of China ; Young Taishan Scholars Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27238]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Wu, Huifeng
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China 3.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhan, Junfei,Wang, Shuang,Li, Fei,et al. Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum[J]. ENVIRONMENTAL POLLUTION,2021,273:9.
APA	Zhan, Junfei,Wang, Shuang,Li, Fei,Ji, Chenglong,&Wu, Huifeng.(2021).Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum.ENVIRONMENTAL POLLUTION,273,9.
MLA	Zhan, Junfei,et al."Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum".ENVIRONMENTAL POLLUTION 273(2021):9.