In recent years, Low-Light-Level (LLL) remote-sensing camera has become a novel subject for the development of aerospace optical remote-sensing payloads. LLL remote sensing camera works in ultra-low light conditions, the image signal is very weak and requires image intensified technology to achieve. In order to make better use of LLL remote sensing data, it is necessary to establish the quantitative relationship between the amount of radiation received by the camera and the digital signal output to process the image. That's radiation calibration technology. Therefore, the radiation calibration of the LLL remote sensing camera is particularly important. In this article, first the requirements for calibration of LLL remote sensing cameras are analyzed in theory. After that, a radiation calibration scheme of the LLL remote sensing camera is put forward. Finally, the radiation calibration test is carried out, and the calibration data are analyzed. The results show that the calibration scheme of LLL remote sensing camera is reasonable and feasible.