Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain | |
Dan, Dan3; Wang, Zhaojun3; Zhou, Xing3; Lei, Ming2,3; Zhao, Tianyu3; Qian, Jia3; Yu, Xianghua3; Yan, Shaohui3; Min, Junwei3; Bianco, Piero1 | |
刊名 | IEEE PHOTONICS JOURNAL |
2021-02 | |
卷号 | 13期号:1 |
关键词 | Super-resolution microscopy structured illumination instant super-resolution imaging spatial domain reconstruction |
ISSN号 | 1943-0655;1943-0647 |
DOI | 10.1109/JPHOT.2021.3053110 |
产权排序 | 1 |
英文摘要 | Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM. We provide a proof-of-principle using mobile fluorescent beads to demonstrate the utility of SDR in imaging moving objects. Consequently, replacement of the FDR approach with SDR significantly enhances SIM as the desired method for live-cell, instant super-resolution imaging. This means that SDR-SIM is a "What You See Is What You Get" approach to super-resolution imaging. |
语种 | 英语 |
出版者 | IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC |
WOS记录号 | WOS:000617366600006 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/94519] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Yao, Baoli |
作者单位 | 1.Univ Nebraska Med Ctr, Coll Pharm, Dept Pharmaceut Sci, Omaha, NE 68198 USA 2.Xi An Jiao Tong Univ, Xian 710049, Peoples R China 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China |
推荐引用方式 GB/T 7714 | Dan, Dan,Wang, Zhaojun,Zhou, Xing,et al. Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain[J]. IEEE PHOTONICS JOURNAL,2021,13(1). |
APA | Dan, Dan.,Wang, Zhaojun.,Zhou, Xing.,Lei, Ming.,Zhao, Tianyu.,...&Yao, Baoli.(2021).Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain.IEEE PHOTONICS JOURNAL,13(1). |
MLA | Dan, Dan,et al."Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain".IEEE PHOTONICS JOURNAL 13.1(2021). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论