Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain
Dan, Dan3; Wang, Zhaojun3; Zhou, Xing3; Lei, Ming2,3; Zhao, Tianyu3; Qian, Jia3; Yu, Xianghua3; Yan, Shaohui3; Min, Junwei3; Bianco, Piero1
刊名IEEE PHOTONICS JOURNAL
2021-02
卷号13期号:1
关键词Super-resolution microscopy structured illumination instant super-resolution imaging spatial domain reconstruction
ISSN号1943-0655;1943-0647
DOI10.1109/JPHOT.2021.3053110
产权排序1
英文摘要

Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM. We provide a proof-of-principle using mobile fluorescent beads to demonstrate the utility of SDR in imaging moving objects. Consequently, replacement of the FDR approach with SDR significantly enhances SIM as the desired method for live-cell, instant super-resolution imaging. This means that SDR-SIM is a "What You See Is What You Get" approach to super-resolution imaging.

语种英语
出版者IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
WOS记录号WOS:000617366600006
内容类型期刊论文
源URL[http://ir.opt.ac.cn/handle/181661/94519]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Yao, Baoli
作者单位1.Univ Nebraska Med Ctr, Coll Pharm, Dept Pharmaceut Sci, Omaha, NE 68198 USA
2.Xi An Jiao Tong Univ, Xian 710049, Peoples R China
3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
推荐引用方式
GB/T 7714
Dan, Dan,Wang, Zhaojun,Zhou, Xing,et al. Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain[J]. IEEE PHOTONICS JOURNAL,2021,13(1).
APA Dan, Dan.,Wang, Zhaojun.,Zhou, Xing.,Lei, Ming.,Zhao, Tianyu.,...&Yao, Baoli.(2021).Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain.IEEE PHOTONICS JOURNAL,13(1).
MLA Dan, Dan,et al."Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain".IEEE PHOTONICS JOURNAL 13.1(2021).
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