Portable fluorescence-based microRNA detection system based on isothermal signal amplification technology | |
Zhang, Zhanying1,3; Wang, Yanfei1,3; Li, Yanlei3; Yu, Dongsheng4; Chen, Haiyan4; Cai, Yi4; Fang, Weikai3; Yang, Zhe4; Ji, Yufeng3,4; Guan, Yifu2,3 | |
刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2019 | |
卷号 | 66期号:1页码:82-90 |
关键词 | fluorescence isothermal rolling-circle amplification miR-183 miRNA |
ISSN号 | 0885-4513 |
DOI | 10.1002/bab.1699 |
英文摘要 | MicroRNAs (miRNAs) diagnostics can be useful for diagnosing or confirming miRNA abundance and are used in screening tests and to assess changes in miRNAs in vivo. At present, the use of traditional nucleic acid amplification assays to detect miRNAs has been limited in laboratory environment because of the time, equipment, and technical expertise required to perform these assays. A specialized, rapid affordable miRNA detection system is necessary when there are limited resources or point-of-care testing needs. We designed a portable and affordable fluorescence-based miRNA detection system based on isothermal signal amplification technology, using SYBR Green II as a fluorescent dye. To reduce costs, we chose LED as a light source and designed the corresponding optical path for LED. The portable detection system shows results consistent with those by real-time PCR, and can be used to detect miR-183 with a limit of detection of approximately 2 fmol. We used the system to detect miR-183 in tissues and blood from patients with hepatocellular carcinoma (HCC). The results from the portable detection device were compared with those from clinical trials and indicated that the miR-183 fluorescence signal could successfully identify HCC and provide information related to cancer progression. (C) 2018 International Union of Biochemistry and Molecular Biology, Inc. |
资助项目 | National Natural Science Foundation of China[81371896] ; Anhui Provincial Natural Science Foundation[1508085SMC214] |
WOS关键词 | SENSITIVE DETECTION |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000458289100010 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/41834] ![]() |
专题 | 医学物理与技术中心 合肥物质科学研究院_中科院安徽光学精密机械研究所 |
通讯作者 | Xu, Chidong |
作者单位 | 1.Univ Sci & Technol China, Hefei, Anhui, Peoples R China 2.China Med Univ, Dept Biochem & Mol Biol, Shenyang, Liaoning, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China 4.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Zhanying,Wang, Yanfei,Li, Yanlei,et al. Portable fluorescence-based microRNA detection system based on isothermal signal amplification technology[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2019,66(1):82-90. |
APA | Zhang, Zhanying.,Wang, Yanfei.,Li, Yanlei.,Yu, Dongsheng.,Chen, Haiyan.,...&Xu, Chidong.(2019).Portable fluorescence-based microRNA detection system based on isothermal signal amplification technology.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,66(1),82-90. |
MLA | Zhang, Zhanying,et al."Portable fluorescence-based microRNA detection system based on isothermal signal amplification technology".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 66.1(2019):82-90. |
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