Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas

doi:10.1039/c8ra01882k

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院等离子体物理研究所

	Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas
	Zhang, Hao 1,5,6; Ma, Jie 1,5; Shen, Jie 1,6; Lan, Yan 1,6; Ding, Lili 5; Qian, Shulou 4; Xia, Weidong 4,5; Cheng, Cheng 1,6; Chu, Paul K.2,3
刊名	RSC ADVANCES
	2018
卷号	8 期号:38 页码:21139-21149
ISSN号	2046-2069
DOI	10.1039/c8ra01882k
通讯作者	Xia, Weidong(xiawd@ustc.edu.cn) ; Cheng, Cheng(chengcheng@ipp.ac.cn) ; Chu, Paul K.(paul.chu@cityu.edu.hk)
英文摘要	Although plasma sterilization has attracted much attention, the underlying mechanisms and biochemical pathways are still not fully understood. In this work, we investigate the molecular mechanism pertaining to the inactivation of Escherichia coli (E. coli) by air discharge plasmas. The membrane protein YgaP and intracellular protein swc7 are over-expressed in E. coli by genetic recombination and gene inducible expression techniques and plasma exposure is demonstrated to alter the structures of YgaP and swc7 in E. coli. The plasma-induced damage of YgaP and swc7 involves changes in the secondary and tertiary structures instead of the primary structure and the modification effectiveness depends on the storage time after the plasma treatment. Owing to the unique structure of E. coli, YgaP is more susceptible to the plasma treatment than intracellular swc7. Within 1 h after plasma exposure, YgaP is modified but not swc7, but after 1 h or longer, both YgaP and swc7 proteins are indeed modified. By analyzing the plasma-induced antimicrobial efficacy and modification of YgaP and swc7, plasma-induced modification of the membrane proteins is the major cause of bacterial death but there is no identifiable relationship with modification of the intracellular protein. The new results provide insights into the mechanism of multiple plasma-induced damage to bacteria and cells as well as the disinfection mechanism.
资助项目	National Natural Science Foundation of China[11675177] ; National Natural Science Foundation of China[11475174] ; National Natural Science Foundation of China[51777206] ; Natural Science Foundation of Anhui Province[1708085MA13] ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)[CityU 11301215] ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)[11205617]
WOS关键词	REACTIVE OXYGEN ; NONTHERMAL PLASMA ; COLD-PLASMA ; ESCHERICHIA-COLI ; CELL-WALL ; INACTIVATION ; STERILIZATION ; JET ; MECHANISMS ; CANCER
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000435576500009
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 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内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/37340]
专题	合肥物质科学研究院_中科院等离子体物理研究所
通讯作者	Xia, Weidong; Cheng, Cheng; Chu, Paul K.
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Hefei 230031, Peoples R China 2.City Univ Hong Kong, Dept Mat Sci & Engn, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China 3.City Univ Hong Kong, Dept Phys, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China 4.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Anhui, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 6.Chinese Acad Sci, Inst Plasma Phys, POB 1126, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hao,Ma, Jie,Shen, Jie,et al. Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas[J]. RSC ADVANCES,2018,8(38):21139-21149.
APA	Zhang, Hao.,Ma, Jie.,Shen, Jie.,Lan, Yan.,Ding, Lili.,...&Chu, Paul K..(2018).Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas.RSC ADVANCES,8(38),21139-21149.
MLA	Zhang, Hao,et al."Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas".RSC ADVANCES 8.38(2018):21139-21149.