Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas | |
Zhang, Hao1,5,6; Ma, Jie1,5; Shen, Jie1,6; Lan, Yan1,6; Ding, Lili5; Qian, Shulou4; Xia, Weidong4,5; Cheng, Cheng1,6; Chu, Paul K.2,3 | |
刊名 | RSC ADVANCES
![]() |
2018 | |
卷号 | 8期号:38页码:21139-21149 |
ISSN号 | 2046-2069 |
DOI | 10.1039/c8ra01882k |
通讯作者 | Xia, Weidong(xiawd@ustc.edu.cn) ; Cheng, Cheng(chengcheng@ipp.ac.cn) ; Chu, Paul K.(paul.chu@cityu.edu.hk) |
英文摘要 | Although plasma sterilization has attracted much attention, the underlying mechanisms and biochemical pathways are still not fully understood. In this work, we investigate the molecular mechanism pertaining to the inactivation of Escherichia coli (E. coli) by air discharge plasmas. The membrane protein YgaP and intracellular protein swc7 are over-expressed in E. coli by genetic recombination and gene inducible expression techniques and plasma exposure is demonstrated to alter the structures of YgaP and swc7 in E. coli. The plasma-induced damage of YgaP and swc7 involves changes in the secondary and tertiary structures instead of the primary structure and the modification effectiveness depends on the storage time after the plasma treatment. Owing to the unique structure of E. coli, YgaP is more susceptible to the plasma treatment than intracellular swc7. Within 1 h after plasma exposure, YgaP is modified but not swc7, but after 1 h or longer, both YgaP and swc7 proteins are indeed modified. By analyzing the plasma-induced antimicrobial efficacy and modification of YgaP and swc7, plasma-induced modification of the membrane proteins is the major cause of bacterial death but there is no identifiable relationship with modification of the intracellular protein. The new results provide insights into the mechanism of multiple plasma-induced damage to bacteria and cells as well as the disinfection mechanism. |
资助项目 | National Natural Science Foundation of China[11675177] ; National Natural Science Foundation of China[11475174] ; National Natural Science Foundation of China[51777206] ; Natural Science Foundation of Anhui Province[1708085MA13] ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)[CityU 11301215] ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)[11205617] |
WOS关键词 | REACTIVE OXYGEN ; NONTHERMAL PLASMA ; COLD-PLASMA ; ESCHERICHIA-COLI ; CELL-WALL ; INACTIVATION ; STERILIZATION ; JET ; MECHANISMS ; CANCER |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000435576500009 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/37340] ![]() |
专题 | 合肥物质科学研究院_中科院等离子体物理研究所 |
通讯作者 | Xia, Weidong; Cheng, Cheng; Chu, Paul K. |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Hefei 230031, Peoples R China 2.City Univ Hong Kong, Dept Mat Sci & Engn, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China 3.City Univ Hong Kong, Dept Phys, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China 4.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Anhui, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 6.Chinese Acad Sci, Inst Plasma Phys, POB 1126, Hefei 230031, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Hao,Ma, Jie,Shen, Jie,et al. Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas[J]. RSC ADVANCES,2018,8(38):21139-21149. |
APA | Zhang, Hao.,Ma, Jie.,Shen, Jie.,Lan, Yan.,Ding, Lili.,...&Chu, Paul K..(2018).Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas.RSC ADVANCES,8(38),21139-21149. |
MLA | Zhang, Hao,et al."Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas".RSC ADVANCES 8.38(2018):21139-21149. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论