NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2

doi:10.1016/j.str.2020.08.008

	NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2
	Zhou, Shu 2,3; Pettersson, Pontus 2; Huang, Jingjing 1; Brzezinski, Peter 2; Pomes, Regis 1; Maler, Lena 2; Adelroth, Pia 2
刊名	STRUCTURE
	2021-03-04
卷号	29
ISSN号	0969-2126
DOI	10.1016/j.str.2020.08.008
通讯作者	Maler, Lena(lena.maler@dbb.su.se) ; Adelroth, Pia(pia.adelroth@dbb.su.se)
英文摘要	The Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it is involved in the formation of supercomplexes composed of cytochrome bc(1) and cytochrome c oxidase. We previously demonstrated that Rcf2 forms a dimer in dodecylphosphocholine micelles, and here we report the solution NMR structure of this Rcf2 dimer. Each Rcf2 monomer has two soluble alpha helices and five putative transmembrane (TM) alpha helices, including an unexpectedly charged TM helix at the C terminus, which mediates dimer formation. The NOE contacts indicate the presence of inter-monomer salt bridges and hydrogen bonds at the dimer interface, which stabilize the Rcf2 dimer structure. Moreover, NMR chemical shift change mapping upon lipid titrations as well as molecular dynamics analysis reveal possible structural changes upon embedding Rcf2 into a native lipid environment. Our results contribute to the understanding of respiratory supercomplex formation and regulation.
资助项目	Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT[653706] ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
WOS关键词	PARTICLE MESH EWALD ; MEMBRANE-PROTEIN ; CHAIN SUPERCOMPLEXES ; SOFTWARE NEWS ; FORCE-FIELD ; SPECTROSCOPY ; ASSIGNMENT ; MODEL ; RCF2 ; C-13
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000629154700009
资助机构	Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/121310]
专题	中国科学院合肥物质科学研究院
通讯作者	Maler, Lena; Adelroth, Pia
作者单位	1.Univ Toronto, Hosp Sick Children, Dept Biochem, Mol Med, Toronto, ON M5G 0A4, Canada 2.Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shu,Pettersson, Pontus,Huang, Jingjing,et al. NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2[J]. STRUCTURE,2021,29.
APA	Zhou, Shu.,Pettersson, Pontus.,Huang, Jingjing.,Brzezinski, Peter.,Pomes, Regis.,...&Adelroth, Pia.(2021).NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.STRUCTURE,29.
MLA	Zhou, Shu,et al."NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2".STRUCTURE 29(2021).