Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli

doi:10.1080/13102818.2021.1885996

	Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli
	Liao, Yingxue 1,3; Ni, Zhijian 1,3; Wu, Jinyong 1,2; Li, Zhongkui 1,3; Ge, Yuanfei 1,3; Chen, Xiangsong 1,2; Yao, Jianming 1,3
刊名	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
	2021
卷号	35
关键词	2&apos -Fucosyllactose Escherichia coli acetate culturing medium induction conditions glucose
ISSN号	1310-2818
DOI	10.1080/13102818.2021.1885996
通讯作者	Chen, Xiangsong(xschen@ipp.ac.cn) ; Yao, Jianming(jmyao@ipp.ac.cn)
英文摘要	2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose-glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2'-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2'-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2'-FL titer reached a maximum value of 7.14 g/L.
资助项目	Science and Technology Service Network Initiative of Chinese Academy of Science[KFJ-STS-QYZD-122]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000621623400001
资助机构	Science and Technology Service Network Initiative of Chinese Academy of Science
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/120655]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Xiangsong; Yao, Jianming
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Plasma Phys, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Inst Plasma Phys, Huainan New Energy Res Ctr, Huainan, Anhui, Peoples R China 3.USTC, Scinece Isl Branch, Grad Sch, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Liao, Yingxue,Ni, Zhijian,Wu, Jinyong,et al. Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2021,35.
APA	Liao, Yingxue.,Ni, Zhijian.,Wu, Jinyong.,Li, Zhongkui.,Ge, Yuanfei.,...&Yao, Jianming.(2021).Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,35.
MLA	Liao, Yingxue,et al."Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 35(2021).