Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma

doi:10.1007/s00432-021-03542-5

	Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma
	Peng, Chanjuan 1,6; Wu, Yong 2,6; Yang, Yang 3,6; Li, Ningning 4,6; Chen, Xi 1,6; Gu, Linhui 5,6; Xu, Dong 1,6; Yang, Chen 1,6
刊名	JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
	2021-02-06
关键词	Ultrasound Microbubbles Ultrasound therapy Radiation therapy Glioblastoma
ISSN号	0171-5216
DOI	10.1007/s00432-021-03542-5
通讯作者	Xu, Dong(xudong@zjcc.org.cn) ; Yang, Chen(yangchen@zjcc.org.cn)
英文摘要	Objective To investigate the efficacy and mechanism of ultrasound-targeted microbubble destruction (UTMD) combined with radiotherapy (XRT) on glioblastoma. Methods The enhanced radiosensitization by UTMD was assessed through colony formation and cell apoptosis in Human glioblastoma cells (U87MG). Subcutaneous transplantation tumors in 24 nude mice implanted with U87MG cells were randomly assigned to 4 different treatment groups (Control, UTMD, XRT, and UTMD + XRT) based on tumor sizes (100-300 mm(3)). Tumor growth was observed for 10 days after treatment, and then histopathology stains (HE, CD34, and gamma H2AX) were applied to the tumor samples. A TUNEL staining experiment was applied to detect the apoptosis rate of mice tumor samples. Meanwhile, tissue proteins were extracted from animal specimens, and the expressions of dsDNA break repair-related proteins from animal specimens were examined by the western blot. Results When the radiotherapy dose was 4 Gy, the colony formation rate of U87MG cells in the UTMD + XRT group was 32 +/- 8%, lower than the XRT group (54 +/- 14%, p < 0.01). The early apoptotic rate of the UTMD + XRT group was 21.1 +/- 3% at 48 h, higher than that of the XRT group (15.2 +/- 4%). The tumor growth curve indicated that the tumor growth was inhibited in the UTMD + XRT group compared with other groups during 10 days of observation. In TUNEL experiment, the apoptotic cells of the UTMD + XRT group were higher than that of the XRT group (p < 0.05). The UTMD + XRT group had the lowest MVD value, but was not significantly different from other groups (p > 0.05). In addition, gamma H2AX increased due to the addition of UTMD to radiotherapy compared to XRT in immunohistochemistry (p < 0.05). Conclusions Our study clearly demonstrated the enhanced destructive effect of UTMD combined with 4 Gy radiotherapy on glioblastoma. This could be partly achieved by the increased ability of DNA damage of tumor cells.
资助项目	National Natural Science Foundation of China[81702968] ; Zhejiang Province Medical and Health Science and Technology Project[2017ZD009] ; Zhejiang Province Medical and Health Science and Technology Project[2019KY334]
WOS研究方向	Oncology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000615551800004
资助机构	National Natural Science Foundation of China ; Zhejiang Province Medical and Health Science and Technology Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/120574]
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, Dong; Yang, Chen
作者单位	1.Chinese Acad Sci, Dept Ultrasound Med, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Dept Med Engn, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Dept Radiat Oncol, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Canc Hosp, Dept Pathol, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Dept Core Facil Serv, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 6.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Peng, Chanjuan,Wu, Yong,Yang, Yang,et al. Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2021.
APA	Peng, Chanjuan.,Wu, Yong.,Yang, Yang.,Li, Ningning.,Chen, Xi.,...&Yang, Chen.(2021).Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY.
MLA	Peng, Chanjuan,et al."Using ultrasound-targeted microbubble destruction to enhance radiotherapy of glioblastoma".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2021).