Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative

doi:10.1016/j.bios.2020.112871

	Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative
	Liu, Xiaolu 2; Xin Lu 3; Tong Zhu 2; Du Wenli 3; Yang Zhenghui 4,5; Cao, Hongzhi 2; Wang, Siyou 6; Tian, Yupeng 3; Zhang, Zhongping 4,5,7; Zhang, Ruilong 3
刊名	BIOSENSORS & BIOELECTRONICS
	2021-03-01
卷号	175
关键词	Lipid droplets ultrastructure Proteohormone simulation BODIPY- Hexylcarbazole derivative STED super-Resolution nanoscopy
ISSN号	0956-5663
DOI	10.1016/j.bios.2020.112871
通讯作者	Tian, Xiaohe(xiaohe.t@ahu.edu.cn)
英文摘要	Lipid droplets (LDs) ultrastructure progression under proteohormone stimulation provide valuable clues for understanding conditions associated with obesity and diabetes. Current available LDs probes either alter intra-LDs environments or lack photo-resistance; thus, this research domain is poorly understood. In this work, a N-B-O type BODIPY- hexylcarbazole derivative named BoCz-Lip was rationally designed while achieving specific LDs live-cell targeting. BoCz-Lip showed minimal impact on cell viability as well as internal LDs' protein, triglyceride and cholesterol level. Along with its good photostability, it can be used to monitor LDs evolution under super-resolution nanoscopy. More importantly, we concluded how proteohormone could influence LDs ultrastructure, offering a better understanding for correlating diseases at the nanoscale.
资助项目	National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[21871003] ; National Natural Science Foundation of China[21701160] ; Anhui University[S020118002/073] ; Anhui University[S020118002/026] ; Natural Science Foundation of Anhui Province of China[1908085MB30] ; Natural Science Foundation of Anhui Province of China[2008085QB52] ; Institute of PhysicalScience and Information Technology, Anhui University ; Doctor Start-up Fund[S020118002/026] ; Hefei National Laboratory for Physical Sciences at the Microscale[KF2019002]
WOS关键词	PERILIPINS ; CELLS ; TIP47 ; PROBE ; DYE
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000607532600003
资助机构	National Natural Science Foundation of China ; Anhui University ; Natural Science Foundation of Anhui Province of China ; Institute of PhysicalScience and Information Technology, Anhui University ; Doctor Start-up Fund ; Hefei National Laboratory for Physical Sciences at the Microscale
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/119877]
专题	中国科学院合肥物质科学研究院
通讯作者	Tian, Xiaohe
作者单位	1.Sichuan Univ, Dept Radiol, Funct & Mol Imaging Key Lab Sichuan Prov, Huaxi MR Res Ctr HMRRC,West China Hosp, Chengdu 610041, England 2.Anhui Univ, Sch Life Sci, Hefei 230601, Peoples R China 3.Anhui Univ, Dept Chem, Key Lab Funct Inorgan Mat Chem Anhui Prov, Hefei 230601, Peoples R China 4.Anhui Univ, Inst Phys Sci, Hefei 230601, Peoples R China 5.Anhui Univ, Inst Informat Technol, Hefei 230601, Peoples R China 6.Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China 7.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei, Peoples R China 8.UCL, London Univ Coll London, Dept Chem, Gower St, London WC1E 6BT, England
推荐引用方式 GB/T 7714	Liu, Xiaolu,Xin Lu,Tong Zhu,et al. Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative[J]. BIOSENSORS & BIOELECTRONICS,2021,175.
APA	Liu, Xiaolu.,Xin Lu.,Tong Zhu.,Du Wenli.,Yang Zhenghui.,...&Tian, Xiaohe.(2021).Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative.BIOSENSORS & BIOELECTRONICS,175.
MLA	Liu, Xiaolu,et al."Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative".BIOSENSORS & BIOELECTRONICS 175(2021).