Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs

doi:10.1186/s13287-020-02004-y

	Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs
	Chen, Guilin 1,3; Zhuo, Yujuan 1,3; Tao, Bo 2,3; Liu, Qian 1,3; Shang, Wenlong 1,3; Li, Yinxiu 1,3; Wang, Yuhong 1,3; Li, Yanli 1,3; Zhang, Lei 4; Fang, Yanwen 5
刊名	STEM CELL RESEARCH & THERAPY
	2020-11-16
卷号	11
关键词	Bone marrow-derived mesenchymal stem cell Static magnetic field Osteoporosis Osteogenic differentiation Magnetic therapy
DOI	10.1186/s13287-020-02004-y
通讯作者	Yu, Ying(yuying@tmu.edu.cn)
英文摘要	Background Osteoporosis is a common metabolic bone disease without effective treatment. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to differentiate into multiple cell types. Increased adipogenic differentiation or reduced osteogenic differentiation of BMSCs might lead to osteoporosis. Whether static magnetic fields (SMFs) might influence the adipo-osteogenic differentiation balance of BMSCs remains unknown. Methods The effects of SMFs on lineage differentiation of BMSCs and development of osteoporosis were determined by various biochemical (RT-PCR and Western blot), morphological (staining and optical microscopy), and micro-CT assays. Bioinformatics analysis was also used to explore the signaling pathways. Results In this study, we found that SMFs (0.2-0.6 T) inhibited the adipogenic differentiation of BMSCs but promoted their osteoblastic differentiation in an intensity-dependent manner. Whole genomic RNA-seq and bioinformatics analysis revealed that SMF (0.6 T) decreased the PPAR gamma-mediated gene expression but increased the RUNX2-mediated gene transcription in BMSCs. Moreover, SMFs markedly alleviated bone mass loss induced by either dexamethasone or all-trans retinoic acid in mice. Conclusions Taken together, our results suggested that SMF-based magnetotherapy might serve as an adjunctive therapeutic option for patients with osteoporosis.
资助项目	National Natural Science Foundation of China[82003827] ; China Postdoctoral Science Foundation[2019 M661040]
WOS关键词	STATIC MAGNETIC-FIELDS ; MESENCHYMAL STEM-CELLS ; PULSED ELECTROMAGNETIC-FIELDS ; WHOLE-BODY EXPOSURE ; PPAR-GAMMA ; MARROW ; OSTEOPOROSIS ; WNT ; MICROCIRCULATION ; OSTEOARTHRITIS
WOS研究方向	Cell Biology ; Research & Experimental Medicine
语种	英语
出版者	BMC
WOS记录号	WOS:000594416800003
资助机构	National Natural Science Foundation of China ; China Postdoctoral Science Foundation
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/105475]
专题	中国科学院合肥物质科学研究院
通讯作者	Yu, Ying
作者单位	1.Tianjin Med Univ, Sch Basic Med Sci, Tianjin Key Lab Inflammatory Biol, Dept Pharmacol, Tianjin 300070, Peoples R China 2.Tianjin Med Univ, Dept Orthoped, Gen Hosp, Tianjin 300070, Peoples R China 3.Tianjin Med Univ, Sch Basic Med Sci, Minist Cosponsored Collaborat Innovat Ctr Med Epi, Tianjin 300070, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 5.Heye Hlth Ind Res Inst Zhejiang Heye Hlth Technol, Anji 313300, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guilin,Zhuo, Yujuan,Tao, Bo,et al. Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs[J]. STEM CELL RESEARCH & THERAPY,2020,11.
APA	Chen, Guilin.,Zhuo, Yujuan.,Tao, Bo.,Liu, Qian.,Shang, Wenlong.,...&Yu, Ying.(2020).Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs.STEM CELL RESEARCH & THERAPY,11.
MLA	Chen, Guilin,et al."Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs".STEM CELL RESEARCH & THERAPY 11(2020).