Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo

doi:10.1016/j.ecoenv.2020.111070

	Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo
	Chen, Biao 3,4; Chai, Qing 5; Xu, Shengmin 1,2; Li, Qian 3,4; Wu, Tao 4; Chen, Shaopeng 4; Wu, Lijun 1,2,3,4
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2020-11-01
卷号	204
关键词	Silver nanoparticles Lung cellular senescence COX2 SASP Human respiratory system
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2020.111070
通讯作者	Xu, Shengmin(shmxu@ahu.edu.cn) ; Wu, Lijun(ljw@ipp.ac.cn)
英文摘要	Silver nanoparticles (AgNPs) are widely used as antimicrobial agents and resulted in their accumulation in environment. The purpose of this study was to investigate the detailed molecular mechanisms underlying AgNP-induced lung cellular senescence which has been proposed as a pathogenic driver of chronic lung disease. Herein, we demonstrate that exposure to AgNPs elevates multiple senescence biomarkers in lung cells, with cell cycle arrest in the G2/M phase, and potently activates genes of the senescence-associated secretory phenotype (SASP) in human fetal lung fibroblast cell line MRCS. Fluorescence-based assay also reveals that apoptosis induced by AgNPs is associated with senescence. Furthermore, we show that AgNPs cause premature senescence through an increase in transcription factor nuclear factor kappa B (NF-kappa B), cyclooxygenase-2 (COX2) expression and overproduction of prostaglandin E2 (PGE2) in lung cells. Inhibition of COX2 reduces AgNPs-induced senescence to a normal level. Moreover, AgNPs also induce upregulation of COX2 and accelerate lung cellular senescence in vivo and cause mild fibrosis in the lung tissue of mice. Taken together, our studies support a critical role of AgNPs in the induction of lung cellular senescence via the upregulation of the COX2/PGE2 intracrine pathway, and suggest the adverse effects to the human respiratory system.
资助项目	National Natural Science Foundation of China[31670860] ; National Natural Science Foundation of China[11835014] ; Recruitment Program for Leading Talent Team of Anhui Province[2019-16] ; National Basic Research 973 Program[2014CB932002] ; Key Program of 13th five-year plan, CASHIPS[KP-2017-05]
WOS关键词	DNA-DAMAGE ; RAT LUNG ; EXPRESSION ; INFLAMMATION ; APOPTOSIS ; CELLS ; IDENTIFICATION ; FIBROBLASTS ; MECHANISMS ; PHENOTYPE
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000574855100002
资助机构	National Natural Science Foundation of China ; Recruitment Program for Leading Talent Team of Anhui Province ; National Basic Research 973 Program ; Key Program of 13th five-year plan, CASHIPS
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/104314]
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, Shengmin; Wu, Lijun
作者单位	1.Anhui Univ, Inst Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Environm Sci & Optoelect Technol, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 5.Qingdao Municipal Ctr Dis Prevent & Control, Clin Lab Microorganism & Parasite, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Chen, Biao,Chai, Qing,Xu, Shengmin,et al. Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,204.
APA	Chen, Biao.,Chai, Qing.,Xu, Shengmin.,Li, Qian.,Wu, Tao.,...&Wu, Lijun.(2020).Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,204.
MLA	Chen, Biao,et al."Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 204(2020).