Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ

doi:10.1016/j.carbpol.2020.115955

	Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ
	Ni, Wenfeng 1,2; Zheng, Zhiming 2; Liu, Hui 2; Wang, Peng 2; Wang, Li 2; Wang, Han 1,2; Sun, Xiaowen 1,2; Yang, Qiang 1,2; Tang, Hengfang 1,2; Zhao, Genhai 2
刊名	CARBOHYDRATE POLYMERS
	2020-05-01
卷号	235
关键词	Carboxymethyl cellulose Magnetic nanoparticles Prenyltransferase NovQ Immobilization Prenylated activity
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2020.115955
通讯作者	Zheng, Zhiming(zhengzhiming2014@163.com) ; Zhao, Genhai(zhgh327@126.com)
英文摘要	Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K-2 using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and Fe3O4 formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found. Molecular docking analysis was also conducted to justify the contribution of multiple amino acids and effect of nanoparticles on catalytic properties of NovQ. Taken together, our study introduces a promising strategy to prepare magnetic nanoparticles and improve the performance of catalyst, which aims for opening new orientations for synthesis of magnetic nanoparticles used for prenyltransferase immobilization.
资助项目	Key research and development plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43] ; China National Key Research and Development Program[2019YFA0904300] ; Major Projects of Science and Technology in Anhui Province[17030801036]
WOS关键词	FE3O4 NANOPARTICLES ; LIPASE ; PURIFICATION ; BIOCATALYST ; ENZYMES
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000519044900022
资助机构	Key research and development plan of Anhui Province ; Natural Science Foundation of Anhui Province ; China National Key Research and Development Program ; Major Projects of Science and Technology in Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103645]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Zhiming; Zhao, Genhai
作者单位	1.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Ni, Wenfeng,Zheng, Zhiming,Liu, Hui,et al. Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ[J]. CARBOHYDRATE POLYMERS,2020,235.
APA	Ni, Wenfeng.,Zheng, Zhiming.,Liu, Hui.,Wang, Peng.,Wang, Li.,...&Zhao, Genhai.(2020).Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ.CARBOHYDRATE POLYMERS,235.
MLA	Ni, Wenfeng,et al."Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ".CARBOHYDRATE POLYMERS 235(2020).