Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer

doi:10.1016/j.tranon.2020.100784

	Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer
	Li, Jianqiang 4,5,6; Li, Xingliang 2,7; Wang, Wenxian 3,4,5; Shao, Yang 1; Zhang, Yiping 3,4,5; Song, Zhengbo 3,4,5
刊名	TRANSLATIONAL ONCOLOGY
	2020-08-01
卷号	13
ISSN号	1936-5233
DOI	10.1016/j.tranon.2020.100784
通讯作者	Song, Zhengbo(songzb@zjcc.org.cn)
英文摘要	OBJECTIVE: This study investigated the feasibility of using malignant pleural effusion (MPE) supernatant and paired cell blocks (precipitate) for gene profiling in patients with non-small cell lung cancer (NSCLC) using next-generation sequencing (NGS) technique. METHODS: Stage IV non-squamous NSCLC patients with MPE were eligible in this prospective study and recruited from Zhejiang Cancer Hospital between May 2014 and October 2015. MPE supernatant and paired precipitate sample gene alterations were determined with NGS containing 14 cancer-related genes. Progression free survival (PFS) was evaluated using Kaplan-Meier method and compared using log-rank test. RESULTS: A total of 102 patients were enrolled in the present study. All pleural effusions were confirmed as malignant with cytological smears. A total of 77 paired MPE supernatant and precipitate samples were acquired from the 102 patients. The results revealed that there were no statistically significant differences in the detection rate and maximum allelic fraction between supernatant and precipitate samples (P = 1.0 and P = .6). Collectively, 172 and 158 genomic alterations with 112 shared mutations were identified in supernatant and precipitate samples, respectively. Comparable PFS was found in FGFR mutation patients according to the supernatant and precipitate sample results (14.0 vs.13.9 months, P = .90). CONCLUSIONS: These results demonstrated that MPE supernatants were comparable to precipitate samples for detection of genetic alteration. However, gene mutation heterogeneity was found between these two media types.
资助项目	National Natural Science Foundation of China[81802276]
WOS关键词	LIQUID BIOPSY ; EGFR MUTATION ; FREE DNA ; CRIZOTINIB ; TISSUE ; NSCLC ; ALK
WOS研究方向	Oncology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000544673300003
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/94808]
专题	中国科学院合肥物质科学研究院
通讯作者	Song, Zhengbo
作者单位	1.Geneseeq Technol Inc, Translat Med Res Ctr, Toronto, ON, Canada 2.Zhejiang Rongjun Hosp, Treatment Ctr, Jiaxing 314000, Zhejiang, Peoples R China 3.Zhejiang Canc Hosp, Dept Med Oncol, Hangzhou 301122, Zhejiang, Peoples R China 4.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Peoples R China 5.Univ Chinese Acad Sci, Dept Thorac Oncol Surg, Canc Hosp, Hangzhou 310022, Peoples R China 6.Zhejiang Canc Hosp, Dept Thorac Oncol Surg, Hangzhou 310022, Peoples R China 7.Zhejiang Rongjun Hosp, Dept Thorac Dis Diag, Jiaxing 314000, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Li, Jianqiang,Li, Xingliang,Wang, Wenxian,et al. Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer[J]. TRANSLATIONAL ONCOLOGY,2020,13.
APA	Li, Jianqiang,Li, Xingliang,Wang, Wenxian,Shao, Yang,Zhang, Yiping,&Song, Zhengbo.(2020).Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer.TRANSLATIONAL ONCOLOGY,13.
MLA	Li, Jianqiang,et al."Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer".TRANSLATIONAL ONCOLOGY 13(2020).