Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets

	Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets
	Zhao, Xian-En; Zuo, Ya-Nan; Qu, Xiaoqing; Sun, Jing; Liu, Lingyuan; Zhu, Shuyun
刊名	MICROCHIMICA ACTA
	2019
卷号	186 期号:12
关键词	Two-dimensional nanomaterials Oxidase mimic Nanozyme Dopamine Hydrogen peroxide 3,3 ',5,5 '-Tetramethylbenzidine Catalytic oxidation Enzyme activities Human serum analysis
英文摘要	The authors describe novel colorimetric assays for tyrosinase (TYR) and catalase (CAT) based on the substrate-triggered decomposition of MnO2 nanosheets (NSs). The MnO2 NSs can act as oxidase mimics that catalyze the oxidation of the substrate tetramethylbenzidine (TMB) to form a blue dye with an absorption maximum at 652 nm. The oxidase-mimicking activity of the MnO2 NSs is inhibited by dopamine (DA)/hydrogen peroxide (H2O2) due to their decomposition of the MnO2 NSs. TYR catalyzes the oxidation of DA while CAT can decompose H2O2 into water and oxygen. Therefore, the oxidase-mimicking activity of MnO2 NSs is restored in the presence of both enzymes and their substrates. Based on the competitive consumption of substrates between enzymes and MnO2 NSs, a colorimetric method for determination of enzyme activity and its substrate is developed. The detection limits for TYR and CAT are 6 mU center dot mL(-1) and 33 mU center dot mL(-1), respectively.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60488]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xian-En,Zuo, Ya-Nan,Qu, Xiaoqing,et al. Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets[J]. MICROCHIMICA ACTA,2019,186(12).
APA	Zhao, Xian-En,Zuo, Ya-Nan,Qu, Xiaoqing,Sun, Jing,Liu, Lingyuan,&Zhu, Shuyun.(2019).Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets.MICROCHIMICA ACTA,186(12).
MLA	Zhao, Xian-En,et al."Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets".MICROCHIMICA ACTA 186.12(2019).