Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin

	Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin
	Dou, Leina; Luo, Linpin; Zhang, Xu; Zhang, Wentao; Liu, Lizhi; Yin, Xuechi; Liu, Sijie; Sun, Jing; Zhang, Daohong; Wang, Jianlong
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2020
卷号	312
关键词	Biomimetic Cell model Liposome Streptolysin O Rhodamine B
英文摘要	Pathogenic bacterial infection has emerged as a global health threat, causing death, disability, and socioeconomic disruption worldwide. The current detection system is generally based on the various characteristics of pathogenic bacteria themselves. The readout method, which relies on the characteristic change of pathogenic bacteria receptor (normal cells), is rarely used. Herein, inspired by the pathogenesis and mimicking a minimalistic model system of living cells, a biomimetic liposome membrane comprising lecithin and cholesterol and encapsulated with rhodamine B (RhB) was innovatively developed to analyze the virulence factor with significantly simplified behavior. Based on the specific interaction between cholesterol and streptolysin 0 (SLO), the biomimetic liposome membrane was selectively ruptured, causing RhB emancipation and enabling fluorescent and intelligent colorimetric dual-signal detections of SLO toxin with limits of detection (LODs) as low as 0.05 mu g.mL(-1) and 0.33 mu gmL(-1), respectively. We anticipate that this study will provide new insights into the development of host cells and biomimetic-system-based methods for pathogen detection.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60329]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dou, Leina,Luo, Linpin,Zhang, Xu,et al. Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,312.
APA	Dou, Leina.,Luo, Linpin.,Zhang, Xu.,Zhang, Wentao.,Liu, Lizhi.,...&Wang, Jianlong.(2020).Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin.SENSORS AND ACTUATORS B-CHEMICAL,312.
MLA	Dou, Leina,et al."Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin".SENSORS AND ACTUATORS B-CHEMICAL 312(2020).