Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s

doi:10.1021/acsami.9b09413

	Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s
	Zhang, Wanlin 1,3; Li, Yao 2; Liang, Yun 1; Yin, Xianpeng 3; Liu, Chengcheng 1; Wang, Shigiang 1; Tian, Li 1; Dong, Hao 1; Li, Guangtao 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2019-08-21
卷号	11 期号:33 页码:30137-30145
关键词	biothiol redox status sensor array poly(ionic liquid) photonic crystal
ISSN号	1944-8244
DOI	10.1021/acsami.9b09413
英文摘要	The three most important redox couples, including cysteine (Cys)/cystine (Cyss), homocysteine (Hcys)/homocystine (Hcyss), and reduced glutathione (GSH)/glutathione disulfide (GSSG), are closely associated with human aging and many diseases. Thus, it is highly important to determine their redox statuses at the following two levels: (i) the redox identity in different thiols/disulfides and (ii) the redox ratio in a mixture of a specific couple. Herein, by using one single AIE-doped (AIE, aggregation-induced emission) photonic-structured poly(ionic liquid) (PIL) sphere as a virtual sensor array, we realize a direct determination of the redox status without a reducing pretreatment of disulfides, which will greatly promote the development of high-throughput and simple procedures. The pattern-recognition method uses the multiple noncovalent interactions of imidazolium-based PILs with these redox species to produce differential responses in both the photonic crystal and fluorescence dual channels. On the one hand, a single sphere enables the direct and simultaneous discrimination of the redox identities of Cys, Cyss, Hcys, Hcyss, GSH, and GSSG under the interference of other five commonly occurring thiols. On the other hand, this sphere also allows for not only a direct quantification of the GSH/GSSG ratios without previously determining the individual concentrations of GSH and GSSG but also the accurate prediction of the ratios in unknown redox samples. To further demonstrate applications of this method, redox mixtures in a biological sample are differentiated. Additionally, quantum calculations further support our assignments for interactions between the imidazolium-based PILs and these redox species.
资助项目	NSFC[21773135] ; NSFC[21473098] ; NSFC[21121004] ; NSFC[21421064] ; MOST[2017YFA0204501] ; MOST[2013CB834502] ; Deutsche Forschungsgemeinschaft DFG[TRR61]
WOS关键词	TURN-ON SENSOR ; FLUORESCENT-PROBES ; SELECTIVE DETECTION ; SENSING PLATFORM ; GLUTATHIONE ; CYSTEINE ; DISCRIMINATION ; ARRAY ; GSH ; QUANTIFICATION
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000482546000069
资助机构	NSFC ; MOST ; Deutsche Forschungsgemeinschaft DFG
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/30829]
专题	中国科学院过程工程研究所
通讯作者	Li, Guangtao
作者单位	1.Tsinghua Univ, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Dept Chem, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 3.Aerosp Res Inst Special Mat & Proc Technol, Beijing 100074, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wanlin,Li, Yao,Liang, Yun,et al. Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(33):30137-30145.
APA	Zhang, Wanlin.,Li, Yao.,Liang, Yun.,Yin, Xianpeng.,Liu, Chengcheng.,...&Li, Guangtao.(2019).Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s.ACS APPLIED MATERIALS & INTERFACES,11(33),30137-30145.
MLA	Zhang, Wanlin,et al."Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s".ACS APPLIED MATERIALS & INTERFACES 11.33(2019):30137-30145.