A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex

doi:10.1126/sciadv.1601217

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物靶标结构与功能中心

	A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex
	Ma, Honglei 1,7; Duan, Jingbo 2,3; Ke, Jiyuan 1,6; He, Yuanzheng 1; Gu, Xin 1; Xu, Ting-Hai 1,7; Yu, Hong 2,3; Wang, Yonghong 2,3; Brunzelle, Joseph S.4; Jiang, Yi7
刊名	SCIENCE ADVANCES
	2017-06
卷号	3 期号:6
ISSN号	2375-2548
DOI	10.1126/sciadv.1601217
文献子类	Article
英文摘要	TOPLESS are tetrameric plant corepressors of the conserved Tup1/Groucho/TLE (transducin-like enhancer of split) family. We show that they interact through their TOPLESS domains (TPDs) with two functionally important ethylene response factor-associated amphiphilic repression (EAR) motifs of the rice strigolactone signaling repressor D53: the universally conserved EAR-3 and the monocot-specific EAR-2. We present the crystal structure of the monocot-specific EAR-2 peptide in complex with the TOPLESS-related protein 2 (TPR2) TPD, in which the EAR-2 motif binds the same TPD groove as jasmonate and auxin signaling repressors but makes additional contacts with a second TPD site to mediate TPD tetramer-tetramer interaction. We validated the functional relevance of the two TPD binding sites in reporter gene assays and in transgenic rice and demonstrate that EAR-2 binding induces TPD oligomerization. Moreover, we demonstrate that the TPD directly binds nucleosomes and the tails of histones H3 and H4. Higher-order assembly of TPD complexes induced by EAR-2 binding markedly stabilizes the nucleosome-TPD interaction. These results establish a new TPD-repressor binding mode that promotes TPD oligomerization and TPD-nucleosome interaction, thus illustrating the initial assembly of a repressor-corepressor-nucleosome complex.
资助项目	Outstanding Young Scientist Foundation of CAS[00000000] ; Youth Innovation Promotion Association of CAS[00000000] ; Van Andel Research Institute[00000000] ; Ministry of Science and Technology (China)[2012ZX09301001] ; Ministry of Science and Technology (China)[2012CB910403] ; Ministry of Science and Technology (China)[2013CB910600] ; Ministry of Science and Technology (China)[XDB08020303] ; Ministry of Science and Technology (China)[2013ZX09507001] ; NSF[91217311] ; NIH[DK071662] ; NIH[GM102545] ; NIH[GM104212] ; NIH[CA181343] ; Michigan Economic Development Corporation[00000000] ; Michigan Technology Tri-Corridor[085P1000817] ; Office of Science of the U. S. Department of Energy[DE-AC02-06CH11357]
WOS关键词	MEDIATED TRANSCRIPTIONAL REPRESSION ; GROUCHO/TRANSDUCIN-LIKE ENHANCER ; CO-REPRESSORS ; EAR MOTIF ; STRUCTURE PREDICTION ; PROTEIN-STRUCTURE ; SHOOT DEVELOPMENT ; PLANT DEVELOPMENT ; STRUCTURAL BASIS ; GENE REPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS记录号	WOS:000406370700001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272623]
专题	药物靶标结构与功能中心
通讯作者	Xu, H. Eric; Li, Jiayang; Melcher, Karsten
作者单位	1.Van Andel Res Inst, Ctr Canc & Cell Biol, 333 Bostwick Ave Northeast, Grand Rapids, MI 49503 USA; 2.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; 4.Northwestern Univ, Synchrotron Res Ctr, Dept Biol Chem & Mol Pharmacol, Life Sci Collaborat Access Team, Argonne, IL 60439 USA; 5.Van Andel Res Inst, Ctr Epigenet, Grand Rapids, MI 49503 USA; 6.NewLink Genet, 2503 South Loop Dr,Suite 5100, Ames, IA 50010 USA 7.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Struct & Funct Drug Targets, VARI SIMM Ctr,Key Lab Receptor Res, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Ma, Honglei,Duan, Jingbo,Ke, Jiyuan,et al. A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex[J]. SCIENCE ADVANCES,2017,3(6).
APA	Ma, Honglei.,Duan, Jingbo.,Ke, Jiyuan.,He, Yuanzheng.,Gu, Xin.,...&Melcher, Karsten.(2017).A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.SCIENCE ADVANCES,3(6).
MLA	Ma, Honglei,et al."A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex".SCIENCE ADVANCES 3.6(2017).